DIFFERENTIAL ORIGINS OF SPINOTHALAMIC TRACT PROJECTIONS TO MEDIAL AND LATERAL THALAMUS IN THE RAT
DIFFERENTIAL ORIGINS OF SPINOTHALAMIC TRACT PROJECTIONS TO MEDIAL AND LATERAL THALAMUS IN THE RAT
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DOI：
10.1002/cne.901840107
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发表时间：
1979-01-01
影响因子：
2.5
通讯作者：
BASBAUM, AI
中科院分区：
文献类型：
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作者：
GIESLER, GJ;MENETREY, D;BASBAUM, AI
在早期的研究中（Giesler等人，1976），发现大鼠脊髓丘脑束（STT）的逆行性起源细胞广泛分布于整个腰椎背角和中间灰质区（IGZ）。STT神经元显然位于腹侧背角和IGZ，这似乎是响应刺激传递到皮下组织，往往只从中线丘脑结构被激活。丘脑外侧结构激活了对伤害性和无害性皮肤刺激作出反应的固有核和边缘区内的STT神经元。用辣根过氧化物酶（HRP）逆行转运研究了丘脑传入神经的这种明显的二分法。在丘脑的各个区域进行单次和多次小剂量HRP注射。组织与二氨基联苯胺（DAB）或邻联茴香胺（OD）反应。DAB组织化学更准确地反映了注射部位周围的HRP浓度，这是可见逆行转运所必需的。OD是检测标记脊髓神经元的更有用的技术。使用这种方法，观察到3-5.3倍的STT神经元，并且更大的形态细节是明显的。充满整个半丘脑的多个HRP灌注标记了背柱核的脊髓延伸内的大量细胞，颈外侧核，腹角和IGZ双侧C[颈]1和C2，以及所有水平的固有核和IGZ。STT神经元也被看到，但在边缘区的数量较少。C3以下脊柱节段的标记是对侧的，只有一个例外。腰膨大的STT神经元比颈膨大的更多。注射到后内侧丘脑结构产生双边标签的腹角和IGZ上颈段。在颈部和腰部扩大，标记的神经元仅限于腹侧背角和IGZ对侧。除了腹角细胞的C1和C2的标记发生显着减少，更多的前内侧丘脑注射产生标记内相同的区域的灰质和数量相媲美的后内侧丘脑注射。注射到丘脑外侧区产生了密集的标签内的尾侧延伸的背柱核和外侧颈核。标记细胞可见于边缘区和固有核的所有水平。大鼠STT可能由2种组分组成，它们的细胞起源、终止和功能很可能不同。
In an earlier study (Giesler et al., 1976), antidromically identified cells of orgin of the rat spinothalamic tract (STT) were found widely distributed throughout the lumbar dorsal horn and intermediate gray zone (IGZ). STT neurons apparently located within the ventral dorsal horn and IGZ, which appeared to respond to stimuli delivered to subcutaneous tissue, tended to be activated only from midline thalamic structures. STT neurons within nucleus proprius and the marginal zone, which responded to noxious and innocuous cutaneous stimuli, were activated from lateral thalamic structures. This apparent dichotomy in thalamic afferents was studied using retrograde transport of horseradish peroxidase (HRP). Single and multiple small injections of HRP were made in various thalamic regions. Tissue was reacted with diaminobenzidine (DAB) or o-dianisidine (OD). DAB histochemistry yielded a more accurate reflection of the HRP concentrations surrounding an injection site which was necessary for visible retrograde transport. OD was the more useful technique for examining labeled spinal cord neurons. With this method, 3-5.3 times more STT neurons were seen and far greater morphological detail was evident. Multiple HRP infusions which filled an entire hemi-thalamus labeled large numbers of cells within the spinal extensions of the dorsal column nuclei, the lateral cervical nucleus, the ventral horns and IGZ bilaterally within C[cervical]1 and C2, and the nucleus proprius and IGZ at all levels. STT neurons were also seen, but in lesser numbers within the marginal zone. Labeling in spinal segments below C3 was, with a single exception, contralateral. The lumbar enlargement contributes a greater number of STT neurons than the cervical enlargement. Injections into posterior medial thalamic structures produced bilateral labeling in the ventral horns and IGZ in upper cervical segments. Within the cervical and lumbar enlargements, labeled neurons were restricted to ventral dorsal horn and to IGZ contralaterally. With the exception of the ventral horn cells of C1 and C2 where a marked reduction in labeling occurred, more anterior medial thalamic injections produced labeling within the identical areas of the gray matter and in numbers comparable to those produced by more posterior medial thalamic injections. Injections into lateral thalamic areas yielded dense labeling within the caudal extensions of the dorsal column nuclei and lateral cervical nucleus. Labeled cells were seen in the marginal zone and nucleus proprius at all levels. The rat STT is probably composed of 2 components which vary in their cells of origin, their terminations and, very likely, their functions.