In vitro activity of taurine-5-bromosalicylaldehyde Schiff base against planktonic and biofilm cultures of methicillin-resistant Staphylococcus aureus.
In vitro activity of taurine-5-bromosalicylaldehyde Schiff base against planktonic and biofilm cultures of methicillin-resistant Staphylococcus aureus.
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DOI：
10.4014/jmb.1401.01037
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发表时间：
2014-08
影响因子：
2.8
通讯作者：
Ruqiang Yuan;Y. Diao;Wenli Zhang;Yuan Lin;Shanshan Huang;Houli Zhang;Li Ma
中科院分区：
文献类型：
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作者：
Ruqiang Yuan;Y. Diao;Wenli Zhang;Yuan Lin;Shanshan Huang;Houli Zhang;Li Ma
金黄色葡萄球菌是一种主要的人类病原体，与社区和医院获得性感染有关。由于耐甲氧西林金黄色葡萄球菌(MRSA)的多重耐药性和强烈的生物被膜特性，其治疗变得越来越困难。席夫碱作为一种很有前途的抗菌剂已经引起了人们的关注。本研究探讨了牛磺酸-5-溴水杨醛Schiff碱(TBSSB)对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性。用微量肉汤稀释法测定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。对浮游型耐甲氧西林金黄色葡萄球菌有较强的抑制作用，最低抑菌浓度为32μg/ml，时间-杀灭曲线证实其对耐甲氧西林金黄色葡萄球菌具有杀菌活性。在24 h时，TBSSB对MRSA生物被膜的形成也有明显的抑制作用，尤其是在1倍MIC和亚MIC水平。此外，扫描电子显微镜和透射电子显微镜显示，接触TBSSB后，MRSA细胞表面的形态和超微结构发生了显著的变化。本研究表明，TBSSB可能是一种有效的杀菌剂。
Staphylococcus aureus is a major human pathogen, implicated in both community and hospital acquired infections. The therapy for methicillin-resistant Staphylococcus aureus (MRSA) infections is becoming more difficult because of multidrug resistance and strong biofilmforming properties. Schiff bases have attracted attention as promising antibacterial agents. In this study, we investigated the in vitro activity of taurine-5-bromosalicylaldehyde Schiff base (TBSSB) against MRSA. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) were determined using a microtiter broth dilution method. TBSSB effectively inhibited planktonic MRSA, with an MIC of 32 μg/ml. The time-kill curve confirmed that TBSSB exhibited bactericidal activity against MRSA. TBSSB was also found to significantly inhibit MRSA biofilm formation at 24 h, especially at 1×MIC and sub-MIC levels. Furthermore, scanning electron microscopy and transmission electron microscopy showed remarkable morphological and ultrastructural changes on the MRSA cell surface, due to exposure to TBSSB. This study indicated that TBSSB may be an effective bactericidal agent against MRSA.