Culture of preimplantation embryos: Facts and artifacts
Culture of preimplantation embryos: Facts and artifacts
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DOI：
10.1093/humupd/1.2.91
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发表时间：
1995-03-01
影响因子：
13.3
通讯作者：
Bavister, BD
中科院分区：
文献类型：
--
作者：
Bavister, BD
哺乳动物植入前胚胎通常在雌性生殖道的保护环境中发育，这实际上排除了原位胚胎发生的研究。因此，必须从培养胚胎的实验中获得信息。因此，对胚胎发生的表观遗传调控的研究长期以来一直与制定能够维持正常发育的培养基的努力交织在一起。在这篇综述中，比较信息胚胎发育的表观遗传调控进行了讨论，包括胚胎的能量底物和氨基酸偏好的信息。简单与复杂的培养基，血清白蛋白或合成的大分子血清替代的优点进行了讨论。共培养的一些潜在的陷阱进行了描述。文化似乎诱导胚胎代谢异常，这可能来自干扰的细胞内pH值。选择终点，以评估实验结果的pesticales被认为是，包括纳入胚胎发育的时间到分析。不良的实验设计和/或数据分析可能会减损甚至否定从胚胎培养中获得的数据的价值;检查实例以帮助纠正这个问题。所有这些点进行了讨论，以期使用数据的需要，胚胎进行改进的培养基的设计，使更高的产量和提高胚胎的生存能力。
Mammalian preimplantation embryos normally develop within the protected environment of the female reproductive tract, which virtually precludes studies on embryogenesis in situ. Information must therefore be derived from experiments on cultured embryos. Consequently, studies on the epigenetic regulation of embryogenesis have long been interwoven with efforts to formulate culture media capable of sustaining normal development. In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos. Advantages of simple versus complex culture media, and of substituting serum albumin or synthetic macromolecules for serum, are discussed. Some potential pitfalls of co-culture are described. Culture appears to induce anomalies in embryo metabolism, which may derive from disturbed intracellular pH. Rationales for selecting endpoints to evaluate the outcome of experiments are considered, including incorporation of timing of embryo development into the analysis. Poor experimental design and/or data analysis can detract from or even negate the value of data obtained from embryo culture; examples are examined to help correct this problem. All of these points are discussed with a view to using data on the needs of embryos for making improvements in the design of culture media, so that higher yields and increased viability of embryos are achieved.