Association of OSMR Gene Polymorphisms with Rheumatoid Arthritis and Systemic Lupus Erythematosus Patients
Association of OSMR Gene Polymorphisms with Rheumatoid Arthritis and Systemic Lupus Erythematosus Patients
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DOI：
10.3109/08916934.2013.849701
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发表时间：
2014-02-01
期刊：
影响因子：
3.5
通讯作者：
Yen, Jeng-Hsien
中科院分区：
文献类型：
--
作者：
Lin, Yuan-Zhao;Li, Ruei-Nian;Yen, Jeng-Hsien
细胞因子参与自身免疫性疾病的发病机制。抑瘤素M受体（OSMR）激活JAK/STAT和MAPK通路，导致多种细胞因子和炎症物质的刺激。许多促炎细胞因子参与类风湿关节炎（RA）和系统性红斑狼疮（SLE）的炎症过程。本研究旨在探讨OSMR基因启动子多态性与RA和SLE患者的关系。研究对象为241例RA患者、143例SLE患者及203名健康人。从外周血单个核细胞中提取基因组DNA，并通过TaqMan实时聚合酶链反应进行基因多态性分型。OMSR启动子区-100 G/T（rs 22922016）基因型与RA易感性无关，但-100 T/T（rs 22922016）基因型可预防干燥综合征和抗Ro抗体的存在。相反，-100 G/T+T/T（rs 22922016）基因型与SLE风险增加显著相关（比值比，OR=1.62，95%置信区间（CI），1.01-2.62）。94.38%的SLE关节炎患者属于-1687C/C（rs 540558）基因型。启动子区-100 T/T（rs 22922016）的T等位基因对RA患者具有保护作用，可改善病情，而该T等位基因是SLE易感性的危险等位基因。类风湿性关节炎和系统性红斑狼疮的疾病严重程度可能部分受到OSMR启动子多态性的影响。
Cytokines are involved in the pathogenesis of autoimmune diseases. Oncostatin M receptor (OSMR) activates JAK/STAT and MAPK pathways leading to the stimulation of a variety of cytokines and inflammatory substances. Many pro-inflammatory cytokines are involved in the inflammatory process of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). In this study, we carried out experiments to examine the relationship of OSMR promoter polymorphisms with RA and SLE patients. 241 patients of RA, 143 patients of SLE and 203 healthy controls were enrolled in their recruitment from the Kaohsiung Medical University Hospital. Genomic DNA was extracted from peripheral blood mononuclear cells and gene polymorphism was genotyped by TaqMan real-time polymerase chain reaction. The OMSR promoter region -100 G/T (rs22922016) genotype was in no relation to the susceptibility of RA, but -100 T/T (rs22922016) genotype could prevent the patients with sicca syndrome and the existence of anti-Ro antibodies. In contrast, the -100 G/T+T/T (rs22922016) genotypes were significantly associated with an increased risk of SLE (odds ratio, OR=1.62, 95% confidence interval (CI), 1.01-2.62). 94.38% of SLE patients with arthritis were belonged to the -1687C/C (rs540558) genotype. The T allele of promoter region -100 T/T (rs22922016) has protective effect and could ameliorate the disease condition in RA patients, whereas the same T allele was a risk allele in the susceptibility of SLE. The disease severity of rheumatoid arthritis and systemic lupus erythematosus can be partially affected by the OSMR promoter polymorphisms.