Characterization and expression analyses of the mouse Wiskott-Aldrich syndrome protein (WASP) family member Wave1/Scar
Characterization and expression analyses of the mouse Wiskott-Aldrich syndrome protein (WASP) family member Wave1/Scar
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DOI：
10.1016/s0378-1119(02)00560-7
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发表时间：
2002-05-15
期刊：
影响因子：
3.5
通讯作者：
Vacher, J
中科院分区：
文献类型：
--
作者：
Benachenhou, N;Massy, I;Vacher, J
参与肌动蛋白重塑和细胞骨架重组的多蛋白复合物的表征对于理解细胞运动和迁移的基本机制至关重要。为了鉴定与这些过程有关的蛋白质，我们分离了小鼠 Wave1/Scar 基因，它是 Wiskott-Aldrich 综合征蛋白 (WASP) 家族的成员。小鼠 Wave1 基因物理定位于 10 号染色体上，跨度超过 12 Kb，包含 8 个外显子和 7 个内含子。小鼠Wave1互补DNA编码预测的559个氨基酸的蛋白质，具有在直系同源蛋白质中保守的SCAR同源结构域、碱性结构域、富含脯氨酸的区域、WASP同源结构域和酸性结构域。 Wave1 转录起始位点位于 ATG 翻译起始位点上游 210 个碱基对处。推定的近端启动子含有推定的 E2 碱性螺旋-环-螺旋转录因子、肝细胞核因子-3β、S8 同源域蛋白、锌指转录因子 MZF-1 和干扰素刺激反应元件的共有结合位点。 Northern分析表明，脑组织中独特的类似于2.6 Kb Wave1转录本的强表达，原位杂交显示小脑浦肯野细胞和海马锥体细胞的有限表达。小鼠 Wave1 基因的表征和表达分析为小鼠模型中 Wave1 在神经元细胞骨架组织中的作用的功能研究提供了基础。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Characterization of multiprotein complexes involved in actin remodeling and cytoskeleton reorganization is essential to understand the basic mechanisms of cell motility and migration. To identify proteins implicated in these processes, we have isolated the mouse Wave1/Scar gene, a member of the Wiskott-Aldrich syndrome protein (WASP) family. The mouse Wave1 gene was physically localized on chromosome 10 and spans over 12 Kb comprising eight exons and seven introns. The mouse Wave1 complementary DNA encodes a predicted 559 amino acid protein, with a SCAR homology domain, a basic domain, a proline-rich region, a WASP homology domain and an acidic domain conserved in the orthologous proteins. The Wave1 transcription initiation site was mapped 210 base pairs upstream of the ATG translational start site. The presumptive proximal promoter contains putative consensus binding sites for E2 basic helix-loop-helix transcription factors, hepatocyte nuclear factor-3beta, S8 homeodomain protein, zinc finger transcription factor MZF-1, and an interferon-stimulated response element. Northern analysis demonstrated a strong expression of a unique similar to2.6 Kb Wave1 transcript in brain tissue, and in situ hybridization showed restricted expression to Purkinje cells from the cerebellum and pyramidal cells from the hippocampus. Characterization and expression analyses of the murine Wave1 gene provide the basis toward functional studies in mouse models of the role of Wave1 in neuronal cytoskeleton organization. (C) 2002 Elsevier Science B.V. All rights reserved.