REPORTER GENE-EXPRESSION IN G2 OF THE 1-CELL MOUSE EMBRYO
REPORTER GENE-EXPRESSION IN G2 OF THE 1-CELL MOUSE EMBRYO
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DOI：
10.1006/dbio.1993.1101
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发表时间：
1993-04-01
影响因子：
2.7
通讯作者：
SCHULTZ, RM
中科院分区：
文献类型：
--
作者：
RAM, PT;SCHULTZ, RM
在 S 早期或中/晚期注射雄性原核后，在 1 细胞小鼠胚胎的 G2 中检测到由 SV40 早期启动子驱动的荧光素酶报告基因的表达量相似。当在 S 早期注射雄性原核并在 S 晚期分析胚胎的表达时，没有观察到荧光素酶活性。最后，当在 S 中/晚期注射雌性原核并分析胚胎的表达时，没有观察到荧光素酶活性。第一个调用周期 G2 中的荧光素酶活性。这些结果表明，合子基因激活在 1 细胞胚胎中启动，而不是像之前认为的那样在 2 细胞胚胎中启动，并且雄性和雌性原核的转录活性之间存在差异。
Similar amounts of expression of a luciferase reporter gene that is driven by the SV40 early promoter are detected in G2 of 1-cell mouse embryo following injection of the male pronucleus in either early or mid/late S. No luciferase activity is observed when the male pronucleus is injected in early S and the embryos analyzed for expression in late S. Last, no luciferase activity is observed when the female pronucleus is injected in mid/late S and the embryos analyzed for luciferase activity in G2 of the first call cycle. These results suggest that zygotic gene activation initiates in the 1-cell embryo rather than in the 2-cell embryo, as previously thought, and that differences between the transcriptional activity of male and female pronuclei exist.