In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products
In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products
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DOI：
10.1016/s0378-4320(99)00030-5
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发表时间：
1999-05-17
影响因子：
2.2
通讯作者：
Wright, RW
中科院分区：
文献类型：
--
作者：
Ellington, JE;Samper, JC;Wright, RW
母马精子（“精子”）库的形成被认为是通过凝集素介导的精子附着在输卵管上皮上而发生的。一旦附着，优选精子的存活受到输卵管因素的支持。种马精子的冷冻保存减少了精子附着在输卵管上皮细胞（OEC）上的数量和这些精子存活的时间。在共培养系统中，精子和OEC之间的体外相互作用的定量可以提供冷冻保存或新鲜精子样品的功能完整性的测定。此外，可从OEC分泌产物中分离出用于体外处理种马精子的上级添加剂。实验1比较了第一次服务的概念（FSC）率导致使用冷冻保存的精子的7种马，精子功能在共培养，如附件OEC和随后的生存时间。根据三个季节观察到的累积FSC率对种马进行分组，在总共217次首次服务（每匹种马31 +/- 9次）中具有平均（44 +/- 3%）或高（65 +/- 2%）生育率。与平均生育力组相比，高生育力组的种马样品具有更多（P = 0.04）的精子附着在OEC上，并且随后的精子在共培养中存活时间更长（P = 0.05）。FSC率与OEC上的精子数量及其在共培养中的存活时间相关（r ≥ 0.71）。在实验2中，冷冻保存的种马精子的功能进行了评估与OEC分泌产物从三个不同来源的文化。培养5小时后，发现与单独在基础TALP培养基中的精子相比，用已被“生物活化”（例如，先前暴露于培养物中的精子）的牛OEC条件培养基孵育的精子更具（P小于或等于0.05）运动性和获能性。精子在这种条件培养基中的存活时间也比在对照培养基中的存活时间长（P = 0.05; 27 +/- 5 h vs. 17 +/- 4 h）。(C)1999 Elsevier Science B. V.保留所有权利。
Formation of a spermatozoa ('sperm') reservoir in the mare is thought to occur through lectin-mediated sperm attachment to the oviductal epithelium. Once attached, prefertilization sperm survival is supported by oviductal factors. Cryopreservation of stallion sperm decreases the number of sperm attaching to oviduct epithelial cells (OEC) and the length of time these sperm survive. Quantification of in vitro interactions between sperm and OEC in a co-culture system may provide an assay for functional integrity of cryopreserved or fresh sperm samples. Additionally, superior additives for in vitro handling of stallion sperm may be isolated from OEC secretory products. Experiment 1 compared first service conception (FSC) rates resulting from the use of cryopreserved sperm of seven stallions, with sperm function in co-culture such as attachment to OEC and subsequent survival time. Stallions were grouped by cumulative FSC rates observed over three seasons as having average (44 +/- 3%) or high (65 +/- 2%) fertility over a total of 217 first services (31 +/- 9 per stallion). Samples from stallions in the high fertility group had more (P = 0.04) sperm attached to OEC and longer subsequent sperm survival in co-culture (P = 0.05) as compared with those from the average fertility group. FSC rates correlated with numbers of sperm attaching to OEC and their survival time in co-culture (r greater than or equal to 0.71). In Experiment 2, the function of cryopreserved stallion sperm was evaluated in culture with OEC secretory products from three different sources. After 5 h of culture, sperm incubated with medium conditioned by bovine OEC which had been 'bioactivated' (e.g. previously exposed to sperm in culture) were found to be more (P less than or equal to 0.05) motile and capacitated as compared to sperm in basal TALP medium alone. Sperm in this conditioned medium also survived longer (P = 0.05; 27 +/- 5 h vs. 17 +/- 4 h) than did those in control medium. (C) 1999 Elsevier Science B.V. All rights reserved.