Effect of Targeted Delivery of Bone Morphogenetic Protein-2 on Bone Formation in Type 1 Diabetes
Effect of Targeted Delivery of Bone Morphogenetic Protein-2 on Bone Formation in Type 1 Diabetes
复制标题
DOI：
10.11607/jomi.3957
复制
发表时间：
2015-05-01
影响因子：
2
通讯作者：
Trackman, Phillip C.
中科院分区：
文献类型：
--
作者：
de Santana, Ronaldo Barcellos;Trackman, Phillip C.
目的：实验性1型糖尿病患者骨形成和愈合减少。本研究调查重组人骨形态发生蛋白2（rhBMP-2）的控制性局部释放是否刺激糖尿病骨缺损愈合，作为其对骨的合成代谢作用的结果。材料和方法：64只BALB/cByJ小鼠在双侧颞骨上造成实验性环形骨缺损。缺损用脱细胞胶原海绵加0.4或1.8 μ g rhBMP-2/缺损处理，未处理的缺损作为对照。在糖尿病和非糖尿病小鼠中，14天内缺损的愈合进行了组织形态计量学分析。结果：糖尿病抑制骨形成在未经处理和BMP治疗的骨缺损。局部释放rhBMP-2可显著刺激糖尿病动物的骨形成，使其接近正常水平，并增强正常动物的骨再生。结论：重组人BMP-2可能对糖尿病膜内骨形成缺陷有治疗作用。
Purpose: Bone formation and healing are diminished in experimental type 1 diabetes. The present study investigated whether controlled local release of recombinant human bone morphogenetic protein 2 (rhBMP-2) stimulates bone defect healing in diabetes as a consequence of its anabolic effects on bone. Materials and Methods: Bilateral experimental circular bone defects were created in the temporal bones of 64 BALB/cByJ mice. Defects were treated with acellular collagen sponge plus 0.4 or 1.8 mu g of rhBMP-2 per defect, and untreated defects served as controls. The healing of the defects over a 14-day period in diabetic and nondiabetic mice was analyzed histomorphometrically. Results: Diabetes inhibited bone formation in both untreated and BMP-treated bone defects. Controlled local release of rhBMP-2 significantly stimulated bone formation in diabetic animals, bringing it nearly to normal levels, and enhanced bone regeneration in normal animals. Conclusion: Recombinant human BMP-2 may be beneficial in treating deficient intramembranous bone formation in diabetes.