Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests
Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests
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DOI：
10.1016/j.cimid.2019.101333
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发表时间：
2019-10-01
影响因子：
2
通讯作者：
Wittek, Thomas
中科院分区：
文献类型：
--
作者：
Lichtmannsperger, Katharina;Hinney, Barbara;Wittek, Thomas
目的：了解G.和C.为了检测奥地利牛中的细小病毒，对来自70个农场的小于180日龄的腹泻小牛的粪便样品进行了检查。在177份粪便样本中，27.1%的贾第鞭毛虫包囊（免疫荧光显微镜）和55.4%的隐孢子虫卵囊（相差显微镜）呈阳性。贾第虫巢式PCR阳性率为83.3%（磷酸丙糖异构酶; tpi）和89.6%（β-贾第虫素; bg），隐孢子虫巢式PCR阳性率为92.5%（60-kDa糖蛋白）。序列分析表明，G.好吧梭parvum可分为IIa家族的4个亚型（IIaA 15 G2 R1，n = 29; IIaA 19 G2 R2，n = 3; IIaA 21 G2 R1，n = 2; IIaA 14 G1 R1，n = 1）。两种免疫层析床旁检测的验证结果分别为29.2%和77.6%的灵敏度;贾第鞭毛虫和隐孢子虫检测的特异性分别为98.4%和91.1%。结果证实了这两种原生动物在奥地利的犊牛中广泛发生。
To obtain information about the occurrence and genotype distribution of G. intestinalis and C. parvum in Austrian cattle, faecal samples from diarrhoeic calves younger than 180 days of age originating from 70 farms were examined. Of the 177 faecal samples, 27.1% were positive for Giardia cysts (immunofluorescence microscopy) and 55.4% for Cryptosporidium oocysts (phase-contrast microscopy). Positive samples were characterized by nested PCR for Giardia, 83.3% (triosephosphate isomerase; tpi) and 89.6% (beta-giardin; bg) were positive, while the Cryptosporidium nested PCR returned 92.5% (60-kDa glycoprotein) positive results. Sequence analysis revealed one assemblage A-positive sample and 30 (bg) respectively 29 (tpi) assemblage E-positive samples for G. intestinalis. For C. parvum four subtypes within the IIa family (IIaA15G2R1, n = 29; IIaA19G2R2, n = 3; IIaA21G2R1, n = 2; IIaA14G1R1, n = 1) could be differentiated. Validation of two immunochromatographic point-of-care tests resulted in a sensitivity of 29.2% and 77.6%; a specificity of 98.4% and 91.1% for the detection of Giardia intestinalis and Cryptosporidium parvum, respectively. Results confirm the widespread occurrence of both protozoa in diarrhoeic calves in Austria.