Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): immunological detection and neuronal localization in insect central nervous system.
Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): immunological detection and neuronal localization in insect central nervous system.
复制标题
神经肽 proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH)：昆虫中枢神经系统的免疫检测和神经元定位。
DOI：
10.1073/pnas.78.9.5899
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发表时间：
1981
影响因子：
11.1
通讯作者：
Miller,RJ
中科院分区：
文献类型：
--
作者：
Bishop,CA;O'Shea,M;Miller,RJ
Proctolin （h - arg - tyrr - leu - pro - throh）是一种首次从蟑螂中提取的五肽。众所周知，它有许多神经激素作用，并与特定的、已识别的蟑螂神经元有关。我们制备了proctolin抗血清，并报道了其在检测蟑螂中枢神经系统proctolin样免疫反应性（PLI）中的应用。放射免疫分析法，能够检测50 fmol的proctolin，用于量化PLI的分布。在生殖神经节中检测到最高浓度，在大脑神经节中检测到最低浓度。蟑螂中枢神经系统免疫组化显示PLI定位于神经元。免疫组化染色的神经元广泛分布于神经节。发现细胞体在动物之间的位置是恒定的，并以双边对称的成对出现。这些神经元是潜在可识别的。
Proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH) is a pentapeptide first extracted from cockroaches. It is known to have many neurohormonal effects and has been associated with specific, identified cockroach neurons. We have produced proctolin antisera and report here on their application in detecting proctolin-like immunoreactivity (PLI) in the cockroach central nervous system. Radioimmunoassay, capable of detectable 50 fmol of proctolin, was used to quantify the distribution of PLI. Highest concentrations were detected in the genital ganglia and lowest in the cerebral ganglia. Immunohistochemistry on the cockroach central nervous system demonstrated that PLI is localized to neurons. Neurons stained by using immunohistochemistry were widespread in the ganglia. Cell bodies were found to be in constant positions from animal to animal and to occur in bilaterally symmetrical pairs. These neurons are potentially identifiable.