Effects of single and mixed species forest ecosystems on diversity and function of soil microbial community in subtropical China
Effects of single and mixed species forest ecosystems on diversity and function of soil microbial community in subtropical China
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中国亚热带单一物种和混交物种森林生态系统对土壤微生物群落多样性和功能的影响
DOI：
10.1007/s11368-011-0442-4
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Liu, Yuanqiu
中科院分区：
文献类型：
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作者：
Jiang, Yumei;Chen, Chengrong;Liu, Yuanqiu
本研究的目的是评估中国亚热带8种单一和混交林生态系统（恢复18年）的土壤微生物群落功能和多样性。从8种森林生态系统的每一种中采集0 - 10厘米深度的土壤样本：马尾松（CP1）；湿地松（CP2）；湿地松和枫香（CBMP1）；湿地松和樟树（CBMP2）；马尾松、枫香和檫木（CBMP3）；枫香和檫木（BMP）；檫木（BP1）；以及枫香（BP2）。通过MicroResp（TM）评估潜在的土壤细菌分解代谢活性，并利用聚合酶链反应（PCR） - 变性梯度凝胶电泳（DGGE）技术分析细菌和真菌群落多样性。阔叶林生态系统（BP2、BMP、BP1）下15种碳（C）源的底物诱导呼吸（SIR）速率和SIR值的分解代谢均匀度远高于针阔混交林生态系统（CBMP1和CBMP2），然后高于纯针叶林生态系统（CP2和CP1）。MicroResp（TM）中的香农 - 韦弗指数值与DGGE方法中的相似，但均匀度值低于DGGE方法中的值。主成分分析使用MicroResp（TM）中的所有15种C源、DGGE图谱中的39条真菌带和21条主要细菌带，揭示了所有土壤样本的分离，表明它们具有不同的潜在C利用模式和不同的微生物群落。第一主成分的得分似乎反映了与不同森林生态系统相关的土壤微生物功能和结构群落的变化。本研究结果表明，森林生态系统显著影响了土壤微生物群落的组成和功能。阔叶树种和针阔混交种植种的土壤微生物群落分解代谢多样性和功能远优于针叶树种。这可能表明阔叶树种下土壤质量恢复的效率高于针叶树种。需要进一步研究以调查本研究中观察到的变化对土壤微生物功能多样性的影响。
The objective of the present study was to evaluate soil microbial community function and diversity among eight single and mixed species forest ecosystems (18-year-old restoration) in subtropical China.Soil samples of the 0-10 cm depth were taken from each of the eight forest ecosystems: Masson pine (CP1); Pitch pine (CP2); Slash pine and Sweetgum (CBMP1); Slash pine and Camphortree (CBMP2); Masson pine and Sweetgum and Chinese Gugertree (CBMP3); Sweetgum and Chinese Gugertree (BMP); Chinese Gugertree (BP1); and Sweetgum (BP2). Potential soil bacterial catabolic activities were assessed by the MicroResp (TM), and bacterial and fungal community diversities were analyzed by using the polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technique.Substrate-induced respiration (SIR) rates and catabolic evenness of the SIR values for 15 carbon (C) sources under the broadleaved forest ecosystems (BP2, BMP, BP1) were much higher than those under the mixed conifer-broadleaved forest ecosystem (CBMP1 and CBMP2), and then under the pure conifer forest ecosystems (CP2 and CP1). Value of Shannon-Weaver index in the MicroResp (TM) was similar to that in the DGGE method, but the value of evenness was lower than that in the DGGE method. Principal component analysis, using all 15 C sources in the MicroResp (TM), 39 bands of fungi and 21 main bands of bacteria in the DGGE profiles, revealed a separation of all the soil samples, indicating that they had different patterns of potential C utilization and different microbial communities. The scores of the first principal component appeared to reflect the variation of soil microbial functional and structural communities associated with different forest ecosystems.Results from this study showed that forest ecosystems have significantly influenced the composition and function of soil microbial community. Catabolic diversity and function of soil microbial community under broadleaf tree species and mixed broadleaf-conifer plant species are much better than under conifer tree species. This may indicate that the efficiency of soil quality restoration is higher under broadleaf species than under coniferous species. Further study is needed for investigating the consequences of the changes seen in this study on soil microbial functional diversity.