Enhanced phytoextraction of an agricultural Cr- and Pb-contaminated soil by bioaugmentation with siderophore-producing bacteria
Enhanced phytoextraction of an agricultural Cr- and Pb-contaminated soil by bioaugmentation with siderophore-producing bacteria
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DOI：
10.1016/j.chemosphere.2008.09.013
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发表时间：
2009-01-01
期刊：
影响因子：
8.8
通讯作者：
Lebeau, Thierry
中科院分区：
文献类型：
--
作者：
Braud, Armelle;Jezequel, Karine;Lebeau, Thierry
由于微生物代谢产物对金属的动员作用更大，生物强化辅助植物提取可以提高植物提取效率。将能够产生铁载体的铜绿假单胞菌、荧光假单胞菌或耐金属罗尔斯通氏菌的绿色荧光蛋白标记的细胞接种到含有Cr（488 mg kg（-1））和Pb（382 mg kg（-1））的农业土壤中，并培养玉米。将细菌作为游离或固定化细胞接种在海藻酸钙珠中，用脱脂牛奶接种，目的是提高细菌的存活率和原位铁载体的产生。添加脱脂乳可增加土壤中接种的假单胞菌浓度。土壤接种R. metallidurans提供脱脂牛奶增加了5.2倍，在玉米芽中的铬积累和接种与固定化铜绿假单胞菌细胞提供脱脂牛奶增加了铬和铅的吸收玉米芽的5.4和3.8倍，分别。然而，整个植物吸收的总金属几乎总是随着生物强化而减少。铜绿假单胞菌或R. metallidurans的6至7倍。土壤中接种细菌的浓度与交换态重金属含量相关。交换态中Cr和Pb含量与地上部或根系中的金属含量相关。我们的研究结果表明，生物强化辅助植物提取是一个相关的方法，旨在提高植物提取率，通常限制了植物修复技术的使用。(C)2008爱思唯尔有限公司保留所有权利。
Bioaugmentation-assisted phytoextraction may enhance the phytoextraction efficiency thanks to larger metal mobilization by microbial metabolites. Green fluorescent protein-tagged cells of Pseudomonas aeruginosa, Pseudo m on as fluorescens or Ralstonia metallidurans, able to produce siderophores, were inoculated in an agricultural soil containing Cr (488 mg kg(-1)) and Pb (382 mg kg(-1)) and maize was cultivated. Bacteria were inoculated as free or immobilized cells in Ca-alginate beads, with skim milk in the aim at improving both the bacterial survival and the in situ siderophore production. Skim milk addition increased inoculated Pseudomonads concentration in soil. Soil inoculation with free cells of R. metallidurans supplied with skim milk increased Cr accumulation in maize shoots by a factor of 5.2 and inoculation with immobilized P. aeruginosa cells supplied with skim milk increased Cr and Pb uptake by maize shoots by a factor of 5.4 and 3.8, respectively. However total metal taken up by the whole plant decreases almost always with bioaugmentation. Translocation factor also increased with P. aeruginosa or R. metallidurans by a factor of 6 up to 7. Inoculated bacteria concentration in soil was correlated with metals in the exchangeable fraction. Cr and Pb concentrations in the exchangeable fraction were correlated with metal contents in shoots or roots. Our results suggest that bioaugmentation-assisted phytoextraction is a relevant method in the aim at increasing the phytoextraction rate which usually limits the use of phytoremediation technologies. (C) 2008 Elsevier Ltd. All rights reserved.