Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization
Detection of Epstein-Barr virus genome within thymic epithelial tumours in Taiwanese patients by nested PCR, PCR in situ hybridization, and RNA in situ hybridization
复制标题
DOI：
10.1002/path.1141
复制
发表时间：
2002-08-01
影响因子：
7.3
通讯作者：
Chiang, H
中科院分区：
文献类型：
--
作者：
Chen, PCH;Pan, CC;Chiang, H
已知EB病毒（EBV）与多种肿瘤相关，包括伯基特淋巴瘤、鼻咽癌和具有类似淋巴上皮瘤样特征的其他器官的一些癌。EB病毒和胸腺上皮肿瘤之间的关联是不确定的，因为在这方面的报告并不完全一致，所采用的方法具有不同的灵敏度和特异性。本研究以78例胸腺瘤及21例胸腺癌为研究对象，从DNA及RNA水平检测病毒基因组。首先通过巢式聚合酶链反应（PCR）靶向第一串联内部重复序列筛选组织块。对阳性病例进一步进行原位PCR原位杂交（ISH）和EB病毒编码RNA-1（EBER-1）ISH定位。胸腺瘤均未检测到EBV基因组。8例胸腺癌巢式PCR EBV阳性，其中6例原位PCR ISH和/或RNA ISH显示肿瘤细胞内的核信号，1例显示淋巴细胞内的信号，1例无明显原位信号。大多数表现为淋巴上皮瘤样形态。这些结果表明，巢式PCR是一种敏感的方法来筛选胸腺上皮肿瘤中的EBV基因组。除EBER-1 RNA ISH外，原位PCR ISH也可用于病毒的定位。胸腺瘤与EB病毒无关，即使在这个流行区。胸腺癌，特别是淋巴上皮瘤样胸腺癌，更经常与病毒有关。版权所有（C）2002约翰威利父子有限公司
Epstein-Barr virus (EBV) is known to be associated with a variety of tumours, including Burkitt's lymphoma, nasopharyngeal carcinoma, and some carcinomas of other organs with similar lymphoepithelioma-like features. The association between EBV and thymic epithelial tumours is inconclusive, as reports in this regard are not entirely consistent and the methods employed are of different sensitivity and specificity. This study examined 78 thymomas and 21 thymic carcinomas in Taiwanese patients, to detect the viral genome at both DNA and RNA levels. The tissue blocks were first screened by nested polymerase chain reaction (PCR) targeting on the first tandem internal repeats. The positive cases were further submitted for viral localization by in situ PCR in situ hybridization (ISH) and Epstein-Barr-encoded RNA-1 (EBER-1) ISH. None of the thymomas showed a detectable EBV genome. Eight thymic carcinomas were positive for EBV by nested PCR, of which six displayed nuclear signals within the tumour cells by in situ PCR ISH and/or RNA ISH, one displayed signals within the lymphocytes, and one showed no discernible in situ signals. Most of them exhibited a lymphoepitbelioma-like morphology. These results show that nested PCR is a sensitive method for screening the EBV genome in thymic epithelial tumours. In situ PCR ISH is reliable for localization of the virus, in addition to EBER-1 RNA ISH. Thymomas are not related to EBV, even in this endemic area. Thymic carcinomas, especially the lymphoepithelioma-like thymic carcinomas, are more often associated with the virus. Copyright (C) 2002 John Wiley Sons, Ltd.