Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - A multi-center study in Taiwan
Association of Mycobacterium tuberculosis genotypes and clinical and epidemiological features - A multi-center study in Taiwan
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DOI：
10.1016/j.meegid.2011.10.001
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Yu, Ming-Chih
中科院分区：
文献类型：
--
作者：
Huang, Shiang-Fen;Su, Wei-Juin;Yu, Ming-Chih
结核分枝杆菌（MTB）的基因型与患者的地理来源和人口迁移有关。结核分枝杆菌基因型与临床表现的关系主要集中在多重耐药株在人群中的传播。本研究旨在探讨台湾地区结核分枝杆菌基因型的分子流行病学、动态变化，以及其与肺结核患者临床表现的关系。进行了一项多中心、为期两年的研究，纳入了516例患者和516株MTB分离株，包括：（1）来自台湾北方的254株分离株;（2）来自台湾中西部的38株分离株;（3）来自台湾南部的211株分离株;和（4）来自台湾东海岸的13株分离株。采用Spoligotyping和标准化的12-loci-MIRU-VNTR方法对分离株进行基因分型。结果显示北京/北京样家系是北方、东部和南部地区MTB的主要基因型，分别占58%、53%和33%。第二个最广泛传播的血统是EAI-马尼拉（20%在西部和南部）和哈勒姆家族（13-27%在南部，西部和东部）。12-MIRU-VNTR基因型的聚类分析表明，北方和南方地区MTB基因型分布存在差异，尤其是26和39位点的12-MIRU-VNTR基因型与隔离年限可能存在时间相关性。此外，部分胸部X线空洞性病变患者与一个北京结核分枝杆菌家族株相关，可通过12-MIRU-VNTR基因型的聚类分析来确定。然而，在纵向研究中，12-loci-MIRU-VNTR基因分型的结果应谨慎解释，因为它的短期不稳定性。不同的分子方法的进一步调查是必要的。(C)2011 Elsevier B. V.保留所有权利。
Genotypes of Mycobacterium tuberculosis (MTB) are related to the geographic origin of the patients and population migration. The relationship between genotypes of MTB and clinical presentations has mainly focused on transmission of multi-drug resistant MTB strain in population. This study aimed to investigate the molecular epidemiology and dynamic change of MTB genotypes in Taiwan, and their association with clinical presentation among patients with pulmonary tuberculosis. A multi-center, two-year study which enrolled 516 patients with 516 MTB isolates was conducted, including: (1) 254 isolates from northern Taiwan; (2)38 isolates from mid-western Taiwan; (3) 211 isolates from southern Taiwan; and (4) 13 isolates from the east coast of Taiwan. The isolates were genotyped with spoligotyping and standardized 12-loci-MIRU-VNTR method. The results showed Beijing/Beijing-like family was the major genotype of MTB in the northern (58%), eastern (53%), and southern (33%) regions. The second most widely spread lineage were the EAI-Manila (20% in the west and south) and Haarlem family (13-27% in the south, west, and east). According to the cluster analysis of 12-MIRU-VNTR genotypes, there were differences in distribution of MTB genotype between the northern and southern regions, and a temporal relationship between isolation year and 12-MIRU-VNTR genotype especially in loci 26 and 39 might exist. Furthermore, some patients with cavity lesions on chest films were associated with a cluster of Beijing family MTB strains, which can be defined by cluster analysis of 12-MIRU-VNTR genotype. However, the results of 12-loci-MIRU-VNTR genotyping in a longitudinal study should be interpreted with caution due to its short term instability. Further investigations of different molecular methodologies are necessary. (C) 2011 Elsevier B.V. All rights reserved.