Decrease of various luteal enzyme activities during prostaglandin F2 alpha-induced luteal regression in bovine.
Decrease of various luteal enzyme activities during prostaglandin F2 alpha-induced luteal regression in bovine.
复制标题
前列腺素 F2 α 诱导牛黄体退化过程中各种黄体酶活性的降低。
DOI：
10.1016/0303-7207(84)90060-1
复制
发表时间：
1984
影响因子：
4.1
通讯作者：
Roche,JF
中科院分区：
文献类型：
--
作者：
Rao,CV;Ireland,JJ;Roche,JF
Luteal gonadotropin receptors decrease in cows, sheep and rats within 24 h following an injection of a luteolytic dose of prostaglandin (PG) F2α. But it is not known whether this decrease is the specific event, or a reflection of general decline in luteal cell structure, function and metabolism. In order to investigate this possibility, 15 of 21 heifers were given on day 9 of the estrous cycle, a single 500 μg injection of Cloprostenol (CO), a synthetic PGF2αanalog. These heifers were ovariectomized in groups of 5 at 12, 24 and 36 h after CO. For controls, a group of 6 heifers were ovariectomized just prior to injection of the others. Serum progesterone levels decreased whereas LH levels increased (P< 0.05) by 12 h with no additional changes observed at 24 or 36 h. The luteal plasma membranes [125I]hCG specific binding, as well as 5'-nucleotidase (5'-NE) activity, decreased by 12 h and continued to decline (P< 0.05) until 24 h (binding) or 36 h (5'-NE). Scatchard analysis showed that the decrease in [125I]hCG binding was due to a decrease in receptor number rather than a decrease in receptor affinity. The activities of cytochrome c oxidase in mitochondria, NADH cytochrome c reductase in rough endoplasmic reticulum and galactosyl transferase in Golgi decreased while NAD pyrophosphorylase in nuclei virtually disappeared following the injection of CO. The β-N-acetyl-D-glucosaminidase (a lysosomal hydrolase) activity in the homogenate increased by 12 h and continued to increase up to 36 h. Glucose 6-phosphate dehydrogenase activity in cytosol was unchanged until 36 h after CO injection. We suggest from the above data that gonadotropin receptor decrease following CO injection is a reflection of general decline in luteal cell structural, functional and metabolic integrity.
