Screening and Characterization of Potential Probiotic Lactic Acid Bacteria from Cultured Common Carp Intestine
Screening and Characterization of Potential Probiotic Lactic Acid Bacteria from Cultured Common Carp Intestine
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DOI：
10.1271/bbb.80746
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发表时间：
2009-07-01
影响因子：
1.6
通讯作者：
Hoshino, Takayuki
中科院分区：
文献类型：
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作者：
Hagi, Tatsuro;Hoshino, Takayuki
本研究季节性地从成年鲤鱼肠道中筛选出具有潜在水产养殖益生菌活性的乳酸菌。分别从夏季和冬季优势乳酸菌中筛选出对胆酸具有抗性且对鱼类病原菌具有较强抗菌活性的乳酸乳球菌h2和棉子乳酸乳球菌h47。还选择了假鸟肠球菌h50，在第1年分离的菌株中具有最强的抗菌活性。从幼鲤肠道优势乳酸菌中筛选出具有较强抗菌活性的海豚链球菌I1。还季节性地进行了具有胆酸抗性的LAB的直接筛选。对分离菌株进行抑菌活性测定，从夏季分离菌株中筛选出一株福中乳杆菌K11。此外，从冬季样品中筛选出5个候选菌株。候选菌株的胆酸抗性和抗菌活性水平优于或至少与其相应的模式菌株相匹配。所有候选者都在很大的温度范围内生长。
Screening of potential probiotic LAB for aquaculture from adult common carp intestine was performed seasonally. Lactococcus lactis h2 and Lactococcus raffinolactis h47, which show cholic acid resistance and strong antibacterial activity against fish pathogens, were selected from predominant LAB in summer and winter respectively. Enterococcus pseudoavium h50, with the strongest antimicrobial activity among the strains isolated through I year, was also selected. Streptococcus iniae I1, with strong antimicrobial activity, was selected from predominant LAB in young common carp intestine. Direct screening of LAB with cholic acid resistance was also carried out seasonally. The antibacterial activity of the isolates was tested, and Lactobacillus fuchuensis K11 was selected from the summer isolates. In addition, five candidate strains were selected from the winter samples. The candidates' levels of cholic acid resistance and antibacterial activity were better than or at the least matched those of their corresponding type strains. All the candidates grew over a wide range of temperatures.