Proteomic analysis of schistosomiasis japonica vaccine candidate antigens recognized by UV-attenuated cercariae-immunized porcine serum IgG2
Proteomic analysis of schistosomiasis japonica vaccine candidate antigens recognized by UV-attenuated cercariae-immunized porcine serum IgG2
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DOI：
10.1007/s00436-013-3447-7
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发表时间：
2013-05
影响因子：
2
通讯作者：
Fang Tian;M. Hou;Lin Chen;Yanan Gao;Xia Zhang;M. Ji;Guanling Wu
中科院分区：
文献类型：
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作者：
Fang Tian;M. Hou;Lin Chen;Yanan Gao;Xia Zhang;M. Ji;Guanling Wu
大量研究表明，辐射减毒尾蚴疫苗可通过细胞和体液机制诱导实验动物对血吸虫感染的高度保护。在这里，我们的目的是通过使用RAC免疫猪或接种和攻击猪的特异性IgG2抗体来鉴定可能的疫苗抗原。用免疫沉淀法从血吸虫可溶性虫体抗原制剂(SWAP)中获得与猪IgG2抗体识别的抗原。用双向电泳法分离了这些抗原，用MALDI-TOF MS从凝胶中约400个推测的斑点中成功地鉴定了116个斑点。在这些斑点中，有113个斑点可以与日本血吸虫匹配。通过基因本体论(GO)数据库对这些蛋白质进行了分类，这些蛋白质的主要功能涉及结合、催化活性(硫氧还蛋白过氧化物酶-2等)、信号转导类(MAP Kinase等)、细胞过程(热休克70-kDa蛋白9B等)和细胞内成分(tektin等)。我们的方法表明，有可能提取特定抗体识别的感兴趣的蛋白质。我们的研究结果可能为探索RAC诱导的高保护性机制提供新的线索，并为抗日本血吸虫病疫苗的研究提供一些启示。
Many studies have showed that the radiation-attenuated cercariae (RAC) vaccine could induce the high protection of laboratory animals to resist the schistosoma infection by cellular and humoral mechanism. Here, we aimed to identify possible vaccine antigens by using specific IgG2 antibody from RAC-vaccinated pigs or vaccination and challenge pigs. The antigens from the schistosomal soluble worm antigen preparation (SWAP) recognized by the porcine IgG2 antibody were obtained using immunoprecipitation technique. These antigens were separated by 2-D electrophoresis, and 116 spots were successfully identified by MALDI-TOF MS from about 400 putative spots in gels. Among these spots, 113 spots could match to theSchistosoma japonicum. These identified proteins in four groups were classified by Gene Ontology (Go) database, and the mainly functions of these proteins were involved in binding, catalytic activity (thioredoxin peroxidase-2, et al.), signal transduction class (MAP Kinase, et al.), cell process (the heat shock 70-kDa protein 9B, et al.), and the intracellular component (tektin, et al.). Our methods suggested that it was possible to pull-down the interesting proteins recognized by specific antibodies. Our results may provide new clues for exploring the mechanism of high protection induced by RAC and shed some light on the research for anti-schistosomiasis japonica vaccine.