LOW TEMPERATURE-INDUCED ALTERATIONS IN THE CHLOROPLAST AND MICROSOMAL-MEMBRANES OF DUNALIELLA-SALINA
LOW TEMPERATURE-INDUCED ALTERATIONS IN THE CHLOROPLAST AND MICROSOMAL-MEMBRANES OF DUNALIELLA-SALINA
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DOI：
10.1104/pp.69.6.1369
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发表时间：
1982-01-01
期刊：
影响因子：
7.4
通讯作者：
THOMPSON, GA
中科院分区：
文献类型：
--
作者：
LYNCH, DV;THOMPSON, GA
利用无细胞壁的单细胞绿色β-D检测膜脂组成的代谢调节。salina（UTEX 1644）作为模型系统。采用低温胁迫启动和研究调控反应。当培养物在30 ℃下生长时，冷却至12 ℃。C，细胞分裂停止约0.4小时。100 h，然后细胞以较慢的速率恢复对数生长。在每种情况下，基于每个细胞的磷脂、糖脂和蛋白质含量均为约0.05。在12 ℃下生长的细胞中高20%。C. 12.degree.的体积。C-驯化的细胞是30 ℃驯化的细胞的2.8倍。C生长的细胞叶绿体膜的数量，如通过形态测定分析确定的，是20%以上，而微粒体膜材料的含量更高，是apprx。2.8是30度的两倍。C生长的细胞对分离自在30 ℃和12 ℃下生长的杜氏藻的纯化叶绿体和微粒体进行脂质组成分析。从30 ℃到12 ℃转变后12和60小时，C.在叶绿体和微粒体磷脂脂肪酸不饱和度增加，在驯化过程中的低温。微粒体磷脂的反应更快，在更大程度上比叶绿体磷脂。尽管有这些变化，磷脂类的相对比例发生在任何细胞组分的变化不大。叶绿体糖脂响应低温显着增加1个特定的类，双半乳糖甘油二酯的比例，相对于单半乳糖甘油二酯，而在任何给定的糖脂类内的脂肪酸分布的变化最小。杜氏藻细胞相对于环境胁迫和完整细胞器的分离可以被操纵的容易性和快速性使得它特别适合于植物细胞内膜间脂质动力学的研究。
The metabolic regulation of membrane lipid composition was examined using the cell wall-less, unicellular green alga D. salina (UTEX 1644) as a model system. Low temperature stress was employed to initiate and study the regulatory response. When cultures growing logarithmically at 30.degree. C were chilled to 12.degree. C, cell division ceased for .apprx. 100 h and then the cells resumed logarithmic growth at a slower rate. The phospholipid, glycolipid and protein content, on a per cell basis, was, in each case, .apprx. 20% higher in cells grown at 12.degree. C. The volume of the 12.degree. C-acclimated cells was 2.8 times that of 30.degree. C-grown cells. The quantity of chloroplast membrane, as determined by morphometric analysis, was 20% greater, whereas the content of microsomal membrane material was more elevated, being .apprx. 2.8 times that of 30.degree. C-grown cells. Lipid compositional analyses were carried out on purified chloroplasts and microsomes isolated from Dunaliella grown at 30 and 12.degree. C and from cells 12 and 60 h following a shift from 30 to 12.degree. C. In chloroplast and microsomal phospholipids fatty acid unsaturation increased during acclimation to low temperature. Microsomal phospholipids responded more quickly and to a greater extent than did chloroplast phospholipids. Despite these alterations, little change in the relative proportions of phospholipid classes occurred in either cell fraction. Chloroplast glycolipids responded to low temperature by significantly increasing the proportion of 1 specific class, digalactosyl diglycerides, relative to monogalactosyl diglycerides, while showing minimal change in fatty acid distribution within any given glycolipid class. The ease and rapidity with which Dunaliella cells can be manipulated with respect to environmental stress and isolation of intact cell organelles makes it particularly well suited for research on intermembrane lipid dynamics within the plant cell.