Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: Modeling, measurements, and implications
Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: Modeling, measurements, and implications
复制标题
DOI：
10.1117/1.1413209
复制
发表时间：
2001-10-01
影响因子：
3.5
通讯作者：
Richards-Kortum, R
中科院分区：
文献类型：
--
作者：
Drezek, R;Sokolov, K;Richards-Kortum, R
目的：在380 nm激发下，宫颈组织荧光光谱显示出随患者年龄和不典型增生的存在而发生的特征性变化。为了定量研究NADH和胶原荧光如何结合组织散射和吸收特性产生测量的组织光谱，开发了蒙特卡罗模型。方法：测定活子宫颈细胞和胶原凝胶幻象的激发-发射基质。对新鲜组织切片进行荧光显微镜检查，以获得荧光团的位置和密度与患者年龄和发育不良的关系。开发了一个蒙特卡罗模型，其中包含了荧光团线形状和空间分布的测量。结果：模型光谱与临床测量结果一致，表明NADH荧光增加和胶原荧光减少在正常和发育不良组织光谱之间产生临床观察到的差异。模型预测对患者年龄和上皮厚度最为敏感。结论：蒙特卡罗技术为研究多个荧光团对测量发射光谱的综合贡献提供了重要手段。随着对体内光学特性的更复杂理解的发展，这种方法将越来越有价值。(C) 2001光学仪器工程师学会。
Objective: At 380 nm excitation, cervical tissue fluorescence spectra demonstrate characteristic changes with both patient age and the presence of dysplasia. A Monte Carlo model was developed in order to quantitatively examine how intrinsic NADH and collagen fluorescence, in combination with tissue scattering and absorption properties, yield measured tissue spectra. Methods: Excitation-emission matrices were measured for live cervical cells and collagen gel phantoms. Fluorescence microscopy of fresh tissue sections was performed to obtain the location and density of fluorophores as a function of patient age and the presence of dysplasia. A Monte Carlo model was developed which incorporated measurements of fluorophore line shapes and spatial distributions. Results: Modeled spectra were consistent with clinical measurements and indicate that an increase in NADH fluorescence and decrease in collagen fluorescence create clinically observed differences between normal and dysplastic tissue spectra. Model predictions were most sensitive to patient age and epithelial thickness. Conclusions: Monte Carlo techniques provide an important means to investigate the combined contributions of multiple fluorophores to measured emission spectra. The approach will prove increasingly valuable as a more sophisticated understanding of in vivo optical properties is developed. (C) 2001 Society of Photo-Optical Instrumentation Engineers.