Identification and characterization of murine DNAM-1 (CD226) and its poliovirus receptor family ligands
Identification and characterization of murine DNAM-1 (CD226) and its poliovirus receptor family ligands
复制标题
DOI：
10.1016/j.bbrc.2005.02.067
复制
发表时间：
2005-04-15
影响因子：
3.1
通讯作者：
Shibuya, A
中科院分区：
文献类型：
--
作者：
Tahara-Hanaoka, S;Miyamoto, A;Shibuya, A
白细胞粘附分子DNAM-1（CD 226）是免疫球蛋白超家族的成员，并且在大多数CD 4+和CD 8 + T淋巴细胞、自然杀伤（NK）细胞、单核细胞/巨噬细胞和B淋巴细胞亚群上组成性表达。脊髓灰质炎病毒受体（PVR; CD 155）及其家族成员连接蛋白2（CD 112）最近已被鉴定为DNAM-1的配体。DNAM-1与配体的相互作用诱导NK细胞和CD 8 + T细胞介导的细胞毒性和细胞因子分泌。然而，受体-配体相互作用的体内功能仍不清楚。在这里，我们确定了小鼠DNAM-1和PVR的同源物，物理和功能上相互结合。我们证明了小鼠DNAM-1的配体结合在抗原特异性CD 8 + T细胞中诱导共刺激信号。这些结果将为探索DNAM-1在体内免疫应答中的作用提供有用的动物模型。(c)2005年爱思唯尔公司All rights reserved.
The leukocyte adhesion molecule DNAM-1 (CD226) is a member of the immunoglobulin superfamily and constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. The poliovirus receptor (PVR; CD155) and its family member nectin 2 (CD112) have recently been identified as the ligands for DNAM-1. Interaction of DNAM-1 with the ligands induces NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. However, in vivo function of the receptor-ligand interaction has remained unclear. Here, we identified murine DNAM-1 and PVR homologues that physically and functionally bind each other. We demonstrated that ligand binding of murine DNAM-1 induced a costimulatory signal in antigen-specific CD8+ T cells. These results should provide a useful animal model to explore a role of DNAM-1 in immune responses in vivo. (c) 2005 Elsevier Inc. All rights reserved.