Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA
Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA
复制标题
使用核酸酶 S1 进行基因组 DNA 的位点选择性切割的人工限制性 DNA 切割器
DOI：
10.1002/cpnc.72
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发表时间：
2019
影响因子：
--
通讯作者：
Makoto Komiyama
中科院分区：
文献类型：
--
作者：
Arivazhagan Rajendran;Narumi Shigi;Jun Sumaoka;Makoto Komiyama
通过将一对伪互补肽核酸（pcPNAs）与S1核酸酶组合，可以获得在预定位点切割DNA的新工具。互补pcPNA侵入DNA双链体并与靶位点的每条链进行碱基配对，产生被S1核酸酶切割的单链区域。切割位点可以通过pcPNA的设计自由调节。这种方法可用于切割人类基因组中的单个位点。该方案提供了使用这种多功能新工具进行位点选择性切割的实验细节。John Wiley & Sons，Inc._关于我们
By combining a pair of pseudo‐complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a target site, creating single‐stranded regions that are cleaved by S1 nuclease. The scission site can be freely modulated by the design of pcPNAs. This method can be used to cleave a single site in the human genome. This protocol presents experimental details for site‐selective scission using this versatile new tool. © 2019 by John Wiley & Sons, Inc.