SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.
SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.
复制标题
DOI：
10.1093/nar/gkh798
复制
发表时间：
2004
影响因子：
14.9
通讯作者：
M. Whitfield;Handan Kaygun;Judith A. Erkmann;W. D. Townley-Tilson;Z. Dominski;W. Marzluff
中科院分区：
文献类型：
--
作者：
M. Whitfield;Handan Kaygun;Judith A. Erkmann;W. D. Townley-Tilson;Z. Dominski;W. Marzluff
茎环结合蛋白（SLBP）结合组蛋白mRNA的3'端，存在于细胞核和细胞质中的多聚核糖体上。SLBP参与组蛋白前体mRNA的加工和成熟信息的翻译。当用DNA复制抑制剂处理细胞时，组蛋白mRNA迅速降解，并通过翻译抑制剂稳定，导致组蛋白mRNA水平增加。在这里，我们表明，SLBP是组蛋白信使核糖核蛋白颗粒（mRNP）的组成部分。来自多聚核糖体的组蛋白mRNA用抗SLBP免疫沉淀。环己酰亚胺处理的细胞中的大多数SLBP存在于多聚核糖体上，这是组蛋白mRNP持续合成和转运到细胞质的结果。当细胞用DNA复制抑制剂处理时，组蛋白mRNA迅速降解，但SLBP水平保持恒定，SLBP重新定位于细胞核。当DNA复制被抑制时，SLBP在RNA结合和组蛋白前mRNA加工中保持活性。
The stem-loop binding protein (SLBP) binds the 3' end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone pre-mRNA and in translation of the mature message. Histone mRNAs are rapidly degraded when cells are treated with inhibitors of DNA replication and are stabilized by inhibitors of translation, resulting in an increase in histone mRNA levels. Here, we show that SLBP is a component of the histone messenger ribonucleoprotein particle (mRNP). Histone mRNA from polyribosomes is immunoprecipitated with anti-SLBP. Most of the SLBP in cycloheximide-treated cells is present on polyribosomes as a result of continued synthesis and transport of the histone mRNP to the cytoplasm. When cells are treated with inhibitors of DNA replication, histone mRNAs are rapidly degraded but SLBP levels remain constant and SLBP is relocalized to the nucleus. SLBP remains active both in RNA binding and histone pre-mRNA processing when DNA replication is inhibited.