Molecular analysis of the mutational effects of Thai street rabies virus with increased virulence in mice after passages in the BHK cell line.
Molecular analysis of the mutational effects of Thai street rabies virus with increased virulence in mice after passages in the BHK cell line.
复制标题
泰国街头狂犬病病毒在 BHK 细胞系中传代后毒力增加的突变效应的分子分析。
DOI：
10.1007/s00705-012-1402-z
复制
发表时间：
2012
影响因子：
2.7
通讯作者：
P. Virojanapirom
中科院分区：
文献类型：
--
作者：
Vigeh M.;Yokoyama K.;Matsukawa T.;Shinohara A.,Ohtani K;P. Virojanapirom
QS-BHK-P7（街道狂犬病病毒）在 BHK 细胞系中传代后，具有与其亲代病毒不同的体外表型。这两种病毒均通过中枢神经系统接种引起小鼠致命感染。然而，只有 QS-BHK-P7 通过肌肉注射途径杀死小鼠。我们发现了四个突变，即糖蛋白 (G) 胞外域中的 S23R 和 H424P、聚合酶基因中的 I1711 V，以及 QS-BHK-P7 磷蛋白和基质蛋白基因之间的非编码区中的另一个突变。先前报道的致病决定簇中没有发生 G 基因的突变。特定非编码区突变的作用仍有待阐明。
QS-BHK-P7, street rabies virus, after passages in the BHK cell line, had anin vitrophenotype that distinguished it from its parental virus. Both viruses caused lethal infection in mice by central nervous system inoculation; however, only QS-BHK-P7 killed mice by the intramuscular route. We found four mutations, S23R and H424P in ectodomain of the glycoprotein (G), I1711 V in the polymerase genes, and another at the non-coding region between the phosphoprotein and matrix protein genes of QS-BHK-P7. None of the mutations in the G gene occurred in previously reported pathogenic determinants. The roles of mutations in particular non-coding regions remain to be elucidated.