F-actin bundles in Drosophila bristles are assembled from modules composed of short filaments.
F-actin bundles in Drosophila bristles are assembled from modules composed of short filaments.
复制标题
DOI：
10.1083/jcb.135.5.1291
复制
发表时间：
1996-12
影响因子：
7.8
通讯作者：
Guild, GM
中科院分区：
文献类型：
--
作者：
Tilney, LG;Connelly, P;Smith, S;Guild, GM
果蝇刚毛中的肌动蛋白束沿着刚毛细胞的长度延伸，因此长度为 65 微米（微毛类）或 400 微米（大毛类），具体取决于刚毛类型。蛹中刚毛伸长完成后不久，随着刚毛表面几丁质化，肌动蛋白束分解。这些包裹以一种奇怪的方式分解；就好像每束被横向锯成平均长度为 3 微米的碎片。肌动蛋白丝的分解在“锯切”表面进行。在所有情况下，相邻束中的切口均以横向对齐方式出现。从这些图像中，我们怀疑每个肌动蛋白束都是由一系列首尾相连的较短的束或模块组成。通过荧光鬼笔环肽染色和连续薄片，我们表明模块化设计存在于非退化束中。用肌球蛋白亚片段 1 装饰同一根刷毛中相邻束中的肌动蛋白丝，表明每个模块中的肌动蛋白丝具有相同的极性。为了研究模块如何发育形成，我们将新形成的和伸长的刷毛切开。刷毛尖端有许多 6-10 根细丝的微小簇。这些簇变得更基础地连接在一起，形成最初组织不良的丝束，但随着时间的推移，它们变得最大限度地交联。然后将额外的细丝添加到这些组织好的束模块的外围。所有这些观察结果使我们意识到肌动蛋白丝束形成和伸长的一种新机制，其中短束被组装并首尾相连地连接到其他短束，就像摩天大楼楼层之间的垂直梁一样。
The actin bundles in Drosophila bristles run the length of the bristle cell and are accordingly 65 microns (microchaetes) or 400 microns (macrochaetes) in length, depending on the bristle type. Shortly after completion of bristle elongation in pupae, the actin bundles break down as the bristle surface becomes chitinized. The bundles break down in a bizarre way; it is as if each bundle is sawed transversely into pieces that average 3 microns in length. Disassembly of the actin filaments proceeds at the "sawed" surfaces. In all cases, the cuts in adjacent bundles appear in transverse register. From these images, we suspected that each actin bundle is made up of a series of shorter bundles or modules that are attached end-to-end. With fluorescent phalloidin staining and serial thin sections, we show that the modular design is present in nondegenerating bundles. Decoration of the actin filaments in adjacent bundles in the same bristle with subfragment 1 of myosin reveals that the actin filaments in every module have the same polarity. To study how modules form developmentally, we sectioned newly formed and elongating bristles. At the bristle tip are numerous tiny clusters of 6-10 filaments. These clusters become connected together more basally to form filament bundles that are poorly organized, initially, but with time become maximally cross-linked. Additional filaments are then added to the periphery of these organized bundle modules. All these observations make us aware of a new mechanism for the formation and elongation of actin filament bundles, one in which short bundles are assembled and attached end-to-end to other short bundles, as are the vertical girders between the floors of a skyscraper.
登录
查看更多内容
DOI：
10.1073/pnas.90.11.4937
发表时间：
1993-06-01
影响因子：
11.1
作者：
APPEL, LF;PROUT, M;FRISTROM, J
通讯作者：
FRISTROM, J
limulus精子的顶体反应中的肌动蛋白丝。通过细丝的包装改变产生的运动。
DOI：
10.1083/jcb.64.2.289
发表时间：
1975-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tilney LG
通讯作者：
Tilney LG
DOI：
10.1083/jcb.130.3.629
发表时间：
1995-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tilney LG;Tilney MS;Guild GM
通讯作者：
Guild GM
影响因子：
7.8
作者：
Cant, K;Knowles, B A;Mooseker, M S;Cooley, L
通讯作者：
Cooley, L
影响因子：
7.8
作者：
SMALL, JV;HERZOG, M;ANDERSON, K
通讯作者：
ANDERSON, K