Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes
Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes
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DOI：
10.1007/s00705-013-1641-7
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发表时间：
2013-07-01
影响因子：
2.7
通讯作者：
Luo, Xuenong
中科院分区：
文献类型：
--
作者：
Li, Huixia;Zhu, Xueliang;Luo, Xuenong
羊口疮病毒（Orf virus，ORFV）是一种具有双链DNA基因组的包膜病毒，主要在山羊和绵羊中引起接触性脓疱性皮炎。本研究从新疆和陕西两地的绵羊和山羊样品中分离到两株ORFV。PCR扩增B2L和病毒干扰素抗性（VIR）基因，并进行序列分析。系统发育分析表明，这两个分离物与其他ORFV株聚在一起，但分为不同的亚群。新疆株与甘肃株同源性最高，陕西株与台湾、湖北株同源性最高。这是中国西北地区首次报道ORFV的分子特征，为中国传染性脓疱皮炎暴发的病原体基因分型提供了新的信息。
Orf virus (ORFV) is an enveloped virus with a double-stranded DNA genome, causing a contagious pustular dermatitis, mainly in goats and sheep. In this study, two strains of ORFV were isolated from sheep and goat samples in Xinjiang and Shaanxi, China. The B2L and virus interferon resistance (VIR) genes of these two isolates were sequenced and analyzed after PCR amplification. Phylogenetic analysis showed that the two isolates clustered with other ORFV strains but were separated into different subgroups. The Xinjiang strain shared the highest homology with the Gansu strain, whereas the Shaanxi strain shared higher homology with the Taiwan and Hubei strains. This is the first report of the molecular characterization of ORFV in Northwest China, and it provides new information on the genotyping of the causative agents responsible for contagious ecthyma dermatitis outbreaks in China.