Sertoli cells of the mouse testis originate from the coelomic epithelium
Sertoli cells of the mouse testis originate from the coelomic epithelium
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DOI：
10.1006/dbio.1998.9068
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发表时间：
1998-11-15
影响因子：
2.7
通讯作者：
Capel, B
中科院分区：
文献类型：
--
作者：
Karl, J;Capel, B
在小鼠的发育过程中，性腺在性交后10.5天（dpc）前不久开始在中肾腹内侧形成。XY性腺由生殖细胞和体细胞组成。生殖细胞的起源已经清楚地确定；然而，体细胞，特别是上皮支持细胞系，即支持细胞的起源尚不清楚。支持细胞是最早在睾丸中分化的体细胞类型，被认为表达雄性性别决定基因Sry，在指导睾丸发育中起着至关重要的作用。以往的研究表明，性腺的体细胞可能起源于中肾小管、中肾间质或体腔上皮。11.5 dpc时的性腺免疫组化染色显示，体腔上皮下的基底膜屏障不连续，提示这一阶段的体腔上皮细胞可能向内移动。为了直接测试这种可能性，使用荧光亲脂染料DiI标记体腔上皮细胞。我们发现，当标记在尾虫15-17期时，对应于11.2-11.4 dpc，两性体腔上皮细胞迁移到性腺。在XY性腺中，迁移的体腔上皮细胞变成了支持细胞和间质细胞。体腔上皮产生支持细胞的能力受到发育调控。在尾体18-20期（11.5-11.7 dpc）标记时，体腔上皮细胞不再成为支持细胞。相反，迁移到性腺的细胞停留在睾丸索外的间质中。在睾丸索形成、体腔上皮下的基膜层增厚形成白膜后，到尾30期（约12.5 dpc）迁移逐渐减少并停止。在XX性腺中，体腔上皮细胞也向性腺迁移，但在同一发育时期没有明显的命运限制。综上所述，我们的数据表明，在发育中的小鼠睾丸中，体腔上皮是支持细胞以及性腺其他体细胞的来源。(C) 1998学术出版社。
During mouse development, the gonad begins to form shortly before 10.5 days postcoitum (dpc) on the ventromedial side of the mesonephros. The XY gonad consists of germ cells and somatic cells. The origin of the germ cells is clearly established; however, the origin of the somatic cells, especially the epithelial supporting cell lineages, called Sertoli cells is still unclear. Sertoli cells are the first somatic cell type to differentiate in the testis and are thought to express Sry, the male sex-determining gene, and to play a crucial role in directing testis development. Previous data have suggested that the somatic cells of the gonad may arise from the mesonephric tubules, the mesonephric mesenchyme, or the coelomic epithelium. Immunohistochemical staining of the gonad at 11.5 dpc showed that the basement membrane barrier under the coelomic epithelium is discontinuous, suggesting that cells in the coelomic epithelium at this stage might move inward. To test this possibility directly, cells of the coelomic epithelium were labeled using the fluorescent lipophilic dye, DiI. We show that when labeled at tail somite 15-17 stages, corresponding to 11.2-11.4 dpc, the coelomic epithelial cells of both sexes migrated into the gonad. In XY gonads, the migrating coelomic epithelial cells became Sertoli cells, as well as interstitial cells. This ability of the coelomic epithelium to give rise to Sertoli cells was developmentally regulated. When labeled at tail somite 18-20 stages, corresponding to 11.5-11.7 dpc, the coelomic epithelial cells no longer became Sertoli cells. Instead, cells that migrated into the gonad stayed outside testis cords, in the interstitium. Migration gradually decreased and ceased by tail somite 30 stage, corresponding to 12.5 dpc, after testis cords had formed and the basement membrane layer underlying the coelomic epithelium had thickened to form the tunica albuginea. In XX gonads, coelomic epithelial cells also migrated into the gonad, but there was no obvious fate restriction during the same developmental period. Taken together, our data show that the coelomic epithelium is a source of Sertoli cells as well as other somatic cells of the gonad in the developing mouse testis. (C) 1998 Academic Press.