Effects of letrozole on hippocampal and cortical catecholaminergic neurotransmitter levels, neural cell adhesion molecule expression and spatial learning and memory in female rats
Effects of letrozole on hippocampal and cortical catecholaminergic neurotransmitter levels, neural cell adhesion molecule expression and spatial learning and memory in female rats
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DOI：
10.1016/j.neuroscience.2007.09.005
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发表时间：
2008-01-02
期刊：
影响因子：
3.3
通讯作者：
Tuzcu, M.
中科院分区：
文献类型：
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作者：
Aydin, M.;Yilmaz, B.;Tuzcu, M.
我们研究了芳香化酶抑制剂来曲唑对雌性大鼠海马体和皮质中的空间学习与记忆、神经细胞黏附分子（NCAM）表达以及儿茶酚胺能神经递质的影响。在完整模型中，成年斯普拉格 - 道利大鼠被分为四组（每组n = 8）。对照组仅接受生理盐水。第二组和第三组动物分别以0.2毫克/千克和1毫克/千克的剂量每日经口灌胃给予来曲唑，持续6周。另一组接受来曲唑治疗的大鼠允许恢复2周。在第二个模型中，24只大鼠被切除卵巢（ovx），第一组作为对照组。第二组接受来曲唑（1毫克/千克）治疗6周。第三组的卵巢切除大鼠给予来曲唑（1微克/千克）加雌二醇（E - 2）（10微克/只大鼠）。最后，所有大鼠在莫里斯水迷宫的空间版本中进行测试。然后将它们断头，迅速取出大脑。儿茶酚胺浓度通过带有电化学检测的高效液相色谱法测定。NCAM 180、140和120异构体通过蛋白质印迹法检测。来曲唑以剂量依赖的方式显著降低子宫重量（P<...（此处原文似乎不完整）
We have investigated effects of letrozole, an aromatase inhibitor, on spatial learning and memory, expression of neural cell adhesion molecules (NCAM) and catecholaminergic neurotransmitters in the hippocampus and cortex of female rats. In the intact model, adult Sprague-Dawley rats were divided into four groups (n=8). Control received saline alone. Letrozole was administered to the animals in the second and third groups by daily oral gavage at 0.2 and 1 mg/kg doses, respectively, for 6 weeks. Another group of letrozole-treated rats was allowed to recover for 2 weeks. In the second model, 24 rats were ovariectomized (ovx) and the first group served as control. The second group received letrozole (1 mg/kg) for 6 weeks. Ovx rats in the third group were given letrozole (1 mu g/kg) plus estradiol (E-2) (10 mu g/rat). At the end, all rats were tested in a spatial version of the Morris water maze. Then they were decapitated and the brains rapidly removed. Catecholamine concentrations were determined by high performance liquid chromatography with electrochemical detection. NCAM 180,140 and 120 isoforms were detected by Western blotting. Uterine weights were significantly reduced by letrozole in a dose-dependent manner (P