Human thymic epithelial cells produce IL-6, granulocyte-monocyte-CSF, and leukemia inhibitory factor.
Human thymic epithelial cells produce IL-6, granulocyte-monocyte-CSF, and leukemia inhibitory factor.
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DOI：
10.4049/jimmunol.145.10.3310
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发表时间：
1990-11
影响因子：
4.4
通讯作者：
P. Le;S. Lazorick;L. Whichard;Yuh Cheng Yang;S. Clark;B. Haynes;K. Singer
中科院分区：
文献类型：
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作者：
P. Le;S. Lazorick;L. Whichard;Yuh Cheng Yang;S. Clark;B. Haynes;K. Singer
培养正常人胸腺上皮（TE）细胞的污染基质细胞的条件的发展，使我们能够表征TE细胞产生的细胞因子的数量。使用人IL-6，粒细胞-单核细胞-CSF和白血病抑制因子（LIF）的cDNA探针，我们通过来自TE细胞的总RNA制剂的RNA印迹分析鉴定了这些细胞因子的mRNA。我们证明TE细胞产生IL-6转录本，TE细胞培养上清液含有IL-6生物活性，如通过支持T1165浆细胞瘤系增殖的能力所确定的。在TE细胞来源的总RNA中也检测到粒细胞-单核细胞-CSF的1.0-磷酸酶（kb）转录物。TE细胞培养物上清液含有LIF活性，如通过鼠细胞系DA-1a的增殖测定的，并且在TE细胞衍生的总RNA制剂中检测到4.0-kb LIF转录物。来自TE细胞来源的总RNA的4.0 kb LIF转录物在大小上对应于PMA活化的T淋巴细胞中的LIF转录物。因此，使用生物测定和RNA印迹分析，我们证明了培养的正常人TE细胞产生免疫调节细胞因子和驱动人造血的各个分化阶段的细胞因子。我们的研究结果支持这一假设，TE细胞可能发挥作用，提供细胞因子是重要的造血前体细胞的增殖和分化，迁移到胸腺在胎儿和出生后的人胸腺发育。
The development of conditions for culturing normal human thymic epithelial (TE) cells free from contaminating stromal cells has allowed us to characterize a number of cytokines produced by TE cells. Using cDNA probes for human IL-6, granulocyte-monocyte-CSF, and leukemia inhibitory factor (LIF), we identified mRNA for these cytokines by RNA blot analysis of total RNA preparations derived from TE cells. We demonstrated that TE cells produced IL-6 transcripts and that TE cell culture supernatants contained IL-6 biologic activity, as determined by the ability to support proliferation of the T1165 plasmacytoma line. The 1.0-kilobase (kb) transcript of granulocyte-monocyte-CSF was also detected in TE cell-derived total RNA. TE cell culture supernatants contained LIF activity, as determined by proliferation of the murine cell line DA-1a, and a 4.0-kb LIF transcript was detected in TE cell-derived total RNA preparations. The 4.0-kb LIF transcript from TE cell-derived total RNA corresponded in size to the LIF transcripts in PMA-activated T lymphocytes. Thus, using biologic assays and RNA blot analysis, we demonstrated that cultured normal human TE cells produced both immunoregulatory cytokines and cytokines that drive various differentiation stages of human hematopoiesis. Our findings support the hypothesis that TE cells may play a role in providing cytokines that are important for the proliferation and differentiation of hematopoietic precursor cells that migrate to the thymus during fetal and postnatal human thymic development.