Plant Tissue Parenchyma and Vascular Bundles Selectively Regulate Stem Cell Mechanosensing and Differentiation
Plant Tissue Parenchyma and Vascular Bundles Selectively Regulate Stem Cell Mechanosensing and Differentiation
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DOI：
10.1007/s12195-022-00737-9
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发表时间：
2022-09
影响因子：
2.8
通讯作者：
K. Driscoll;Maya S. Butani;Kirstene A. Gultian;Abigail McSweeny;Jay M. Patel;Sebastián L. Vega
中科院分区：
文献类型：
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作者：
K. Driscoll;Maya S. Butani;Kirstene A. Gultian;Abigail McSweeny;Jay M. Patel;Sebastián L. Vega
植物组织丰富多样，由于趋同进化，在结构上与许多动物组织相似。脱细胞植物组织具有类似于松质骨（多孔薄壁组织）和骨骼肌（纤维维管束）的显微形貌。然而，使用植物组织作为一种廉价和丰富的生物材料控制干细胞behaviors.MethodsCelery植物组织被切断横截面（多孔薄壁组织）或纵向（纤维维管束）和脱细胞。人类间充质干细胞（MSCs），然后培养顶部植物组织和单细胞的共聚焦成像被用来评估微地形对MSC粘附，形态，细胞骨架排列，是相关蛋白（雅普）信号传导，和下游谱系承诺成骨或成肌phenotypes.ResultsMicrotopography的早期影响是保守的植物组织脱细胞后，MSCs附着和植物组织上增殖。在多孔薄壁组织上培养的MSCs沿植物组织孔周围沿着各向同性分布。相比之下，在维管束上培养的MSC在纤维状维管束方向上各向异性地伸展并排列。微地形的差异也影响MSC核雅普定位和肌动蛋白各向异性，在纤维组织中观察到更高的值。当暴露于成骨或成肌培养基时，多孔实质上的MSC具有较高百分比的细胞对骨生物标志物碱性磷酸酶染色阳性，而成肌细胞决定蛋白1（MyoD）对于纤维血管束上的MSC显著上调。和差异化。
IntroductionPlant tissues are plentiful, diverse, and due to convergent evolution are structurally similar to many animal tissues. Decellularized plant tissues feature microtopographies that resemble cancellous bone (porous parenchyma) and skeletal muscle (fibrous vascular bundles). However, the use of plant tissues as an inexpensive and abundant biomaterial for controlling stem cell behavior has not been widely explored.MethodsCelery plant tissues were cut cross-sectionally (porous parenchyma) or longitudinally (fibrous vascular bundles) and decellularized. Human mesenchymal stem cells (MSCs) were then cultured atop plant tissues and confocal imaging of single cells was used to evaluate the early effects of microtopography on MSC adhesion, morphology, cytoskeletal alignment, Yes-associated protein (YAP) signaling, and downstream lineage commitment to osteogenic or myogenic phenotypes.ResultsMicrotopography was conserved post plant tissue decellularization and MSCs attached and proliferated on plant tissues. MSCs cultured on porous parenchyma spread isotropically along the periphery of plant tissue pores. In contrast, MSCs cultured on vascular bundles spread anisotropically and aligned in the direction of fibrous vascular bundles. Differences in microtopography also influenced MSC nuclear YAP localization and actin anisotropy, with higher values observed on fibrous tissues. When exposed to osteogenic or myogenic culture medium, MSCs on porous parenchyma had a higher percentage of cells stain positive for bone biomarker alkaline phosphatase, whereas myoblast determination protein 1 (MyoD) was significantly upregulated for MSCs on fibrous vascular bundles.ConclusionsTogether, these results show that plant tissues are an abundant biomaterial with defined microarchitecture that can reproducibly regulate MSC morphology, mechanosensing, and differentiation.