Induction of ornithine decarboxylase, RNA, and protein synthesis in macrophage cell lines stimulated by immunoadjuvants.
Induction of ornithine decarboxylase, RNA, and protein synthesis in macrophage cell lines stimulated by immunoadjuvants.
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免疫佐剂刺激的巨噬细胞系中鸟氨酸脱羧酶、RNA 和蛋白质合成的诱导。
DOI：
10.1002/jcp.1041200111
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发表时间：
1984
影响因子：
5.6
通讯作者：
Nichols,WK
中科院分区：
文献类型：
--
作者：
Prosser,FH;Schmidt,CJ;Nichols,SV;Nichols,WK
使用两种小鼠巨噬细胞系PU 5 - 1.8和J774.1研究了与巨噬细胞蛋白合成的佐剂刺激相关的早期生化变化。将PU 5 - 1.8和J774.1细胞暴露于两种粗免疫佐剂BCG细胞壁（BCGcw）和大肠杆菌脂多糖（LPS）后2小时，检测到鸟氨酸脱羧酶（ODC）的诱导。化学上确定的免疫佐剂糖肽，N-乙酰基-胞壁酰-L-丙氨酰-D-异谷氨酰胺（MDPL）也在2小时时促进ODC活性的增加，在4小时后达到最大，而没有佐剂活性的D-丙氨酰类似物（MDPD）几乎没有观察到作用。在PU 5 - 1.8和J774.1细胞中，BCG cw促进的ODC活性增加在6至8小时后恢复至对照水平。BCGcw还刺激RNA和蛋白质合成，其在至少24小时内保持升高，并且与DNA合成和细胞增殖的减少相关。BCGcw和MDPL诱导的ODC在两种细胞系中均被加入PGE 2所增强。吲哚美辛轻微降低了J774.1细胞中BCGcw对ODC刺激的幅度，但未能改变PU 5 - 1.8细胞的反应。额外的观察结果表明，在两种细胞系中，BCGcw诱导ODC之前，都激活了环AMP依赖性蛋白激酶。这些观察结果表明，环AMP介导的ODC诱导可能是巨噬细胞中佐剂刺激的早期生化标志物。
Early biochemical changes associated with adjuvant stimulation of macrophage protein synthesis were studied using two murine macrophage cell lines, PU5‐1.8 and J774.1. An induction of ornithine decarboxylase (ODC) was detected 2 hours after exposure of PU5‐1.8 and J774.1 cells to two crude immunoadjuvants, BCG cell walls (BCGcw) and lipopolysaccharides fromEscherichia coli(LPS). The chemically defined immunoadjuvant glycopeptide, N‐acetyl‐muramyl‐L‐alanyl‐D‐isoglutamine (MDPL) also promoted an increase in ODC activity at 2 hours that was maximal after 4 hours, while little or no effect was observed with the D‐alanyl analog (MDPD) that is devoid of adjuvant activity. The increase in ODC activity promoted by BCGcw in PU5‐1.8 and J774.1 cells returned toward control levels by 6 to 8 hours. BCGcw also stimulated RNA and protein synthesis which remained elevated for at least 24 hours and was associated with a decrease in DNA synthesis and cell proliferation. ODC induction by BCGcw and MDPLwas enhanced by the addition of PGE2in both cell lines. Indomethacin slightly depressed the magnitude of ODC stimulation by BCGcw in J774.1 cells but failed to alter the response of PU5‐1.8 cells. Additional observations indicated that the induction of ODC by BCGcw in both cell lines was preceded by an activation of cyclic AMP‐dependent protein kinase. These observations suggest that a cyclic AMP‐mediated induction of ODC may be an early biochemical marker of adjuvant stimulation in macrophages.