Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: Intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein
Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: Intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein
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DOI：
10.1002/j.1460-2075.1996.tb00656.x
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发表时间：
1996-06-17
期刊：
影响因子：
11.4
通讯作者：
Gerisch, G
中科院分区：
文献类型：
--
作者：
Hanakam, F;Albrecht, R;Gerisch, G
嗜组蛋白是富含组氨酸残基的肉豆蔻酰化蛋白，已知其以质膜结合和可溶性胞质状态存在于网骨藻细胞中。使用嗜组蛋白与绿色荧光蛋白（GFP）的融合物和共聚焦激光扫描显微镜监测这些蛋白响应于pH变化的细胞内易位，正常和突变的非豆蔻酰化融合蛋白都以pH依赖性的方式在细胞内穿梭。降低pH后，这些蛋白在几分钟内在细胞质、质膜和细胞核之间移位。组氨酸簇的表面上的hisactophilin分子的蛋白质结合到质膜和在其转移到细胞核的作用进行了讨论的基础上的pH开关机制。
Hisactophilins are myristoylated proteins that are rich in histidine residues and known to exist in Dictyostelium cells in a plasma membrane-bound and a soluble cytoplasmic state, Intracellular translocation of these proteins in response to pH changes was monitored using hisactophilin fusions with green fluorescent protein (GFP) and confocal laser scanning microscopy, Both the normal and a mutated non-myristoylated fusion protein shuttled within the cells in a pH-dependent manner, After lowering the pH, these proteins translocated within minutes between the cytoplasm, the plasma membrane and the nucleus. The role of histidine clusters on the surface of hisactophilin molecules in binding of the proteins to the plasma membrane and in their transfer to the nucleus is discussed on the basis of a pH switch mechanism.