Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography
Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography
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DOI：
10.1073/pnas.1911262116
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发表时间：
2019-12-10
影响因子：
11.1
通讯作者：
Daum, Bertram
中科院分区：
文献类型：
--
作者：
Gambelli, Lavinia;Meyer, Benjamin H.;Daum, Bertram
表面蛋白层（S层）通常是古细菌细胞壁的唯一结构组成部分，因此对细胞存活非常重要。S层具有多种细胞功能，包括维持细胞形状、渗透和机械稳定性、在细胞周围形成半渗透性保护屏障、细胞-细胞相互作用以及表面粘附。尽管S层对古生物至关重要，但它们的三维（3D）结构仍然知之甚少。在这里，我们提出了详细的三维电子低温显微镜地图的古菌S-层从3个不同的硫化叶菌菌株。我们能够精确定位并确定2个亚基SlaA和SlaB的结构。我们还提出了一个模型，描述了成熟的S层的组装。
Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell-cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer.