Biocompatibility of poly(ethylene glycol) and poly(acrylic acid) interpenetrating network hydrogel by intrastromal implantation in rabbit cornea.
Biocompatibility of poly(ethylene glycol) and poly(acrylic acid) interpenetrating network hydrogel by intrastromal implantation in rabbit cornea.
复制标题
兔角膜基质内植入聚乙二醇和聚丙烯酸互穿网络水凝胶的生物相容性。
DOI：
10.1002/jbm.a.35453
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ta,ChristopherN
中科院分区：
文献类型：
--
作者：
Zheng,LuoLuo;Vanchinathan,Vijay;Dalal,Roopa;Noolandi,Jaan;Waters,DaleJ;Hartmann,Laura;Cochran,JenniferR;Frank,CurtisW;Yu,CharlesQ;Ta,ChristopherN
我们在兔模型中评价了聚乙二醇和聚丙烯酸（PEG/PAA）互穿网络水凝胶的生物相容性。将直径为6 mm的PEG/PAA水凝胶植入12只兔的角膜基质中。基质瓣用微型角膜刀制作。随机分配6只家兔承受植入物2个月，2只家兔承受植入物6个月，2只家兔承受植入物9个月，1只家兔承受植入物12个月，1只家兔承受植入物16个月。每月对家兔进行评价。在指定的时间段后，摘除眼睛，并处理角膜进行组织学和免疫组织化学。植入水凝胶2个月的6只家兔中有3只角膜透明。植入6个月或更长时间的6只家兔中，4只角膜保持透明。这6只兔角膜上皮缺损和变薄的发生率较高。一只计划9个月的家兔在4个月时发生植入物挤出。16个月家兔的角膜保持透明，但组织学显示上皮向内生长。PEG/PAA的基质内植入导致长期并发症的发生率较高。© 2015 Wiley Periodicals，Inc. J Biomed Mater Res Part A：103A：3157-3165，2015.
We evaluated the biocompatibility of a poly(ethylene glycol) and poly(acrylic acid) (PEG/PAA) interpenetrating network hydrogel designed for artificial cornea in a rabbit model. PEG/PAA hydrogel measuring 6 mm in diameter was implanted in the corneal stroma of twelve rabbits. Stromal flaps were created with a microkeratome. Randomly, six rabbits were assigned to bear the implant for 2 months, two rabbits for 6 months, two rabbits for 9 months, one rabbit for 12 months, and one rabbit for 16 months. Rabbits were evaluated monthly. After the assigned period, eyes were enucleated, and corneas were processed for histology and immunohistochemistry. There were clear corneas in three of six rabbits that had implantation of hydrogel for 2 months. In the six rabbits with implant for 6 months or longer, the corneas remained clear in four. There was a high rate of epithelial defect and corneal thinning in these six rabbits. One planned 9‐month rabbit developed extrusion of implant at 4 months. The cornea remained clear in the 16‐month rabbit but histology revealed epithelial in‐growth. Intrastromal implantation of PEG/PAA resulted in a high rate of long‐term complications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3157–3165, 2015.