Eukaryotic RNA polymerase I promoter binding is directed by protein contacts with transcription initiation factor and is DNA sequence-independent.
Eukaryotic RNA polymerase I promoter binding is directed by protein contacts with transcription initiation factor and is DNA sequence-independent.
复制标题
真核 RNA 聚合酶 I 启动子的结合是通过蛋白质与转录起始因子的接触来指导的，并且与 DNA 序列无关。
DOI：
10.1016/0092-8674(87)90327-8
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发表时间：
1987
期刊：
影响因子：
64.5
通讯作者：
Paule,MR
中科院分区：
文献类型：
--
作者：
Kownin,P;Bateman,E;Paule,MR
通过一系列间隔区突变体的体外转录和足迹实验，检测了RNA聚合酶I与真核细胞核糖体RNA基因启动子-转录起始因子(TIF)复合体的结合。聚合酶有效地与TIF-启动子复合体结合，不依赖于聚合酶相互作用区的DNA序列，并在富含AT的模板上启动TIF结合位点下游的固定距离的转录。甲基丙基-EDTAFe(Li)足迹法证实了聚合酶和DNA之间的最小接触。我们推测，聚合酶是通过DNA序列无关的机制直接指向启动子的，仅通过与TIF的蛋白质-蛋白质接触。结合之后的起始步骤需要转录起始部位区域中的特殊序列特征。
RNA polymerase I binding to the eukaryotic ribosomal RNA gene promoter-transcription initiation factor (TIF) complex was examined by in vitro transcription and footprinting of a series of spacer mutants. Polymerase binds efficiently to the TIF-promoter complex independently of the DNA sequence in the polymerase interaction region and initiates transcription a fixed dista’nce downstream of the TIF binding site on AT-rich templates. Methidlumpropyl-EDTAFe (lI) footprinting confirms minimal contacts between polymerase and DNA. We infer that polymerase is directed to the promoter by a DNA sequence-independent mechanism, solely by protein-protein contacts with TIF. An initiation step subsequent to binding requires special sequence characteristics in the transcription start site region.