Expression of zebrafish ROR alpha gene in cerebellar‐like structures
Expression of zebrafish ROR alpha gene in cerebellar‐like structures
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DOI：
10.1002/dvdy.21275
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发表时间：
2007-09
影响因子：
2.5
通讯作者：
Y. Katsuyama;Yoshihiro Oomiya;Hideyuki Dekimoto;Eriko Motooka;Ai Takano;S. Kikkawa;M. Hibi;T. Terashima
中科院分区：
文献类型：
--
作者：
Y. Katsuyama;Yoshihiro Oomiya;Hideyuki Dekimoto;Eriko Motooka;Ai Takano;S. Kikkawa;M. Hibi;T. Terashima
小鼠遗传学研究已经确定了几个与小脑发育有关的基因。小鼠突变体Stagerer和Lurcher分别缺乏维甲酸相关孤儿受体α(RORα)和谷氨酸受体Delta2(GRID2)基因，它们在小脑中表现出相似的功能和发育异常。本文报道了斑马鱼RORα同源基因ROA1和ROA2的克隆和表达模式，并与GRID2的表达模式进行了比较。ROA1和ROA2的表达分别在原肠发育晚期和咽发育后期开始。ROA1和ROA2在视网膜和顶盖均有空间表达。与哺乳动物的RORα一样，在小脑中也观察到了ROA2的表达。在成人大脑中，ROA2和GRID2在大脑区域共表达，这些区域被称为小脑样结构。这些观察表明，脊椎动物大脑中的RORα同源基因在进化上具有保守的功能。发展动力学236：2694-2701,2007。©2007 Wiley-Liss Inc.
Mouse genetic studies have identified several genes involved in cerebellar development. The mouse mutants staggerer and lurcher are functionally deficient for the retinoid‐related orphan receptor alpha (ROR alpha) and glutamate receptor delta2 (Grid2) genes, respectively, and they show similar functional and developmental abnormalities in the cerebellum. Here, we report the cloning and expression pattern of zebrafish ROR alpha orthologues rora1 and rora2, and compare their expression pattern with that of grid2. Expression of rora1 and rora2 is initiated at late gastrula and pharyngula stages, respectively. Both rora1 and rora2 are spatially expressed in the retina and tectum. Expression of rora2 was further observed in the cerebellum, as reported for mammalian ROR alpha. In the adult brain, rora2 and grid2 are coexpressed in brain regions, designated as cerebellar‐like structures. These observations suggest an evolutionarily conserved function of ROR alpha orthologues in the vertebrate brain. Developmental Dynamics 236:2694–2701, 2007. © 2007 Wiley‐Liss, Inc.