Elizabethkingia anophelis, an emerging pathogen, inhibits RAW 264.7 macrophage function
Elizabethkingia anophelis, an emerging pathogen, inhibits RAW 264.7 macrophage function
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DOI：
10.1111/1348-0421.12888
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发表时间：
2021-07-16
影响因子：
2.6
通讯作者：
Matsushita, Osamu
中科院分区：
文献类型：
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作者：
Mayura, I. Putu Bayu;Gotoh, Kazuyoshi;Matsushita, Osamu
按蚊嗜酸菌是一种病原体，可导致免疫功能低下的患者发生危及生命的感染。第一例E. 2013年报告了按蚊感染;随后，全球报告其发病率增加。此外，在2015年美国疫情中观察到死亡率超过30%。迄今为止，E.按蚊感染，如毒素生产，仍然不清楚。由于组织巨噬细胞是抵抗病原体的第一道防线，因此在本研究中，E。按蚊和巨噬细胞样细胞系RAW 264.7。虽然E.按蚊对RAW 264.7巨噬细胞没有细胞毒性，感染抑制了LPS诱导的形态学变化和RAW 264.7巨噬细胞向M1样表型极化的分化标志物的激活。然而，当使用Transwell插入物限制细胞接触或使用细菌培养上清液代替活细菌时，未观察到这种抑制。此外，还证明了E.按蚊逃避吞噬作用。总的来说，结果表明，E.按蚊感染以接触依赖性方式抑制RAW 264.7巨噬细胞分化为促炎表型。
Elizabethkingia anophelis is a pathogen that can cause a life-threatening infection in immunocompromised patients. The first case of E. anophelis infection was reported in 2013; subsequently, an increase in its incidence has been reported globally. Additionally, a mortality rate of more than 30% was observed in the US outbreak of 2015. To date, the pathogenic mechanisms underlying E. anophelis infection, such as toxin production, remain unclear. Since tissue macrophages act as the first line of defense against pathogens, in the present study the interactions between E. anophelis and a macrophage-like cell line RAW 264.7 were examined. Although E. anophelis showed no cytotoxicity toward RAW 264.7 macrophages, the infection inhibited LPS-induced morphological changes and activation of differentiation markers for the polarization of RAW 264.7 macrophages toward an M1-like phenotype. However, when the cell contact was restricted using Transwell inserts or bacterial culture supernatants were used instead of live bacteria, no such inhibition was observed. Moreover, it was shown that E. anophelis evaded phagocytosis. Overall, the results suggest that E. anophelis infection inhibits the differentiation of RAW 264.7 macrophages to a pro-inflammatory phenotype in a contact-dependent manner.