Correlation study between the pharmacokinetics of seven main active ingredients of Mahuang decoction and its pharmacodynamics in asthmatic rats
Correlation study between the pharmacokinetics of seven main active ingredients of Mahuang decoction and its pharmacodynamics in asthmatic rats
复制标题
麻黄汤七种主要活性成分在哮喘大鼠体内药动学与药效学的相关性研究
DOI：
10.1016/j.jpba.2020.113144
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发表时间：
2020-05-10
影响因子：
3.4
通讯作者：
He, Yu
中科院分区：
文献类型：
--
作者：
Huang, Pengcheng;Tang, Yinghong;He, Yu
The aim of this study was to investigate the pharmacokinetics and pharmacodynamics of seven main active components of Mahuang decoction (MHD) and its time-concentration-effect relationship. The asthmatic rat model was established by the method of ovalbumin (OVA) sensttization. The plasma concentrations of ephedrine, pseudoephedrine, methylephedrine, amygdalin, liquiritin, cinnamic acid, glycyrrhizic acid in asthmatic model rat were investigated by a selective and rapid HPLC/MS-MS method. Simultaneously, the asthma-involved cytokines including leukotrienes B-4 (LTB4), thromboxane B-2 (TXB2), 6-Keto-Prostaglandin F-1 alpha (6-K-PGF(1 alpha)) and histamine (HIS) levels in rat plasma were determined by using ELISA. A mathematics method was applied to assess the trend of percentage rate of change among different time intervals of the seven components. The sigmoid E max function was used to establish the PK-PD modeling of MHD. The results indicated that MHD could control or ameliorate asthma. There was a hysteresis between the peaked drug concentration and maximum therapeutic effect of MHD. The PK-PD curves of MHD showed clockwise or counter-clockwise hysteresis loop. In addition, amygdalin might exert a more significant influence on regulating cytokines levels in asthmatic rats among the seven components of MHD. (C) 2020 Elsevier B.V. All rights reserved.
