INFLUENCES OF METAL-IONS ON THE REACTION OF AMINO AND IMINO ACIDS WITH FLUOROGENIC REAGENTS
INFLUENCES OF METAL-IONS ON THE REACTION OF AMINO AND IMINO ACIDS WITH FLUOROGENIC REAGENTS
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DOI：
10.1016/0003-2697(85)90450-6
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
NAKAJIMA, T
中科院分区：
文献类型：
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作者：
MIYANO, H;TOYOOKA, T;NAKAJIMA, T
当两倍过量的金属离子将（Cu 2+、Ni 2+、Zn 2+、Mg 2+、Ca 2+、Fe 3+和Al 3+）添加到氨基酸的反应介质中（各1-50 μ M）与荧光试剂NBD-F（4-氟-7-硝基苯并-2-氧杂-1，3-二唑），DNS-Cl（5-N，N-二甲基氨基萘磺酰氯）、OPA（邻苯二甲醛）和荧光胺（4-苯基螺[呋喃-2（3 H），1“-邻苯二甲酸]-3，3”-二酮），观察到衍生化减少。在与NBD-F的反应中，效果尤其显著。Cu ~（2+）和/或Ni ~（2+）抑制DNS-Cl和荧光胺对组氨酸和天冬氨酸的衍生化作用，抑制OPA对组氨酸的衍生化作用。通过向反应介质中加入EDTA可以克服金属离子的抑制。为了测定少量的氨基酸和亚氨基酸（小于μ M），建议在用这些亲电试剂衍生化之前向生物样品中加入螯合试剂。
When a twofold excess of metal ions (Cu2+,Ni2+, Zn2+,Mg2+,Ca2+,Fe3+, and Al3+) was added to a reaction medium of amino acids (1-50 .mu.M each) with the fluorogenic reagents NBD-F (4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole), DNS-Cl (5-N,N-dimethylaminonaphthalenesulfonyl chloride), OPA (o-phthalaldehyde), and fluorescamine (4-phenylspiro[furan-2(3H),1''-phthalan]-3,3''-dione), a reduction of derivatization was observed. The effect was especially marked on the reaction with NBD-F. The derivatization of His and Asp with DNS-Cl and fluorescamine and of His with OPA was suppressed by Cu2+ and/or Ni2+. The suppression by the metal ions can be overcome by the addition of EDTA to the reaction medium. For determination of a small amount of amino and imino acids (less than .mu.M) the addition of a chelating reagent to biological samples is recommended before derivatization with these electrophilic reagents.