An expanded view of bacterial DNA replication
An expanded view of bacterial DNA replication
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DOI：
10.1073/pnas.122040799
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发表时间：
2002-06-11
影响因子：
11.1
通讯作者：
Noirot, P
中科院分区：
文献类型：
--
作者：
Noirot-Gros, MF;Dervyn, E;Noirot, P
通过全基因组双杂交筛选和系统特异性分析，构建了一个以枯草芽孢杆菌复制机制为中心的蛋白质相互作用网络。该网络由连接69个蛋白质的91个特异性相互作用组成。超过四分之一的相互作用发生在已知在其他生物中相互作用的蛋白质的同源物之间，这表明我们报告的其他相互作用具有很高的生物学意义。这些相互作用提供了对DNA复制与重组和修复、膜结合蛋白复合物和信号通路的关系的见解。它们还导致未知蛋白质的生物学作用，如高度保守的YabA所示，它在这里显示为在起始控制中起作用。因此，我们的相互作用图谱为发现细菌DNA复制的各个方面提供了一个有价值的工具。
A protein-interaction network centered on the replication machinery of Bacillus subtilis was generated by genome-wide two-hybrid screens and systematic specificity assays. The network consists of 91 specific interactions linking 69 proteins. Over one fourth of the interactions take place between homologues of proteins known to interact in other organisms, indicating the high biological significance of the other interactions we report. These interactions provide insights on the relations of DNA replication with recombination and repair, membrane-bound protein complexes, and signaling pathways. They also lead to the biological role of unknown proteins, as illustrated for the highly conserved YabA, which is shown here to act in initiation control. Thus, our interaction map provides a valuable tool for the discovery of aspects of bacterial DNA replication.