Multidrug-resistance-associated protein MGr1-Ag is identical to the human 37-kDa laminin receptor precursor
Multidrug-resistance-associated protein MGr1-Ag is identical to the human 37-kDa laminin receptor precursor
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DOI：
10.1007/s00018-002-8531-6
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发表时间：
2002-09-01
影响因子：
8
通讯作者：
Fan, D
中科院分区：
文献类型：
--
作者：
Shi, Y;Zhai, H;Fan, D
我们报告的分离和功能特性的基因编码MGr 1-Ag，多药耐药相关蛋白。用单克隆抗体MGr 1筛选大肠癌SW 480细胞的cDNA文库。22个阳性克隆（命名为R1-R22）的DNA同源性分析表明，人37-kDa层粘连蛋白受体前体（37 LRP，R7/R9/R15/R16/R19/R20）和一个新基因（R22）为编码MGr 1-Ag的候选基因。Western blot分析显示，抗R20血清与MGr 1-Ag反应，而抗R22血清与MGr 1-Ag不反应。的编码基因。采用逆转录-PCR扩增MGr 1-Ag。序列分析表明，MGr 1-Ag基因与37 LRP基因的编码序列相同。体外药物敏感性实验表明，通过反义技术下调37 LRP可显著增强抗癌药物对胃癌细胞的杀伤作用。因此，我们得出结论，MGr 1-Ag是相同的37 LRP。
We report the isolation and functional characterization of the gene encoding MGr 1-Ag, a multidrug-resistance-associated protein. A lambdagt11 cDNA library derived from colorectal carcinoma SW480 cells was screened with monoclonal antibody MGr1. DNA homology analysis of 22 positive clones (designated R1-R22) suggested human 37-kDa laminin receptor precursor (37LRP, R7/R9/R15/R16/R19/R20) and a novel gene (R22) as candidate genes encoding MGr1-Ag. Western blot analysis showed that anti-R20 serum reacted with a unique protein band that was consistent with MGr1-Ag, while anti-R22 serum could not react with MGr1 -Ag. The coding gene for. MGr1-Ag was amplified using reverse transcription-PCR. Sequence analysis revealed that the MGr1-Ag and 37LRP genes shared the same coding sequence. An in vitro drug sensitivity assay indicated that down-regulation of 37LRP by an antisense technique could significantly enhance the cytotoxicity of anticancer drugs to gastric cancer cells. Thus we draw the conclusion that MGr1-Ag is identical to 37LRP.