Enhanced rhamnolipids production by Pseudomonas aeruginosa based on a pH stage-controlled fed-batch fermentation process
Enhanced rhamnolipids production by Pseudomonas aeruginosa based on a pH stage-controlled fed-batch fermentation process
复制标题
基于 pH 阶段控制的补料分批发酵工艺提高铜绿假单胞菌的鼠李糖脂产量
DOI：
10.1016/j.biortech.2012.04.091
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发表时间：
2012-08-01
影响因子：
11.4
通讯作者：
Lu, Wenyu
中科院分区：
文献类型：
--
作者：
Zhu, Lingqing;Yang, Xue;Lu, Wenyu
鼠李糖脂因其低毒、高生物降解性和环境安全性而被广泛用作天然表面活性剂、乳化剂和抗生素。本研究建立了铜绿假单胞菌O-2-2发酵鼠李糖脂的pH阶段性控制工艺。通过对搅拌速度的优化，在5-L发酵罐上进行了分批发酵，发酵得率为24.06g/L。通过控制pH，鼠李糖脂产量提高到28.8g/L，提高了19.7%，更多的底物转化为鼠李糖脂而不是生物量。基于pH分级控制发酵的细胞生长、产物合成和底物消耗的发酵动力学模型表明，补料分批发酵可以进一步提高鼠李糖脂的产量。在pH级控制的补料分批发酵中，当搅拌速度控制在500rpm，发酵温度保持在30℃时，鼠李糖脂的产量达到了70.56g/L，提高了193%。
Rhamnolipids find broad applications as natural surfactants, emulsifiers, and antibiotics because of their low toxicity, high biodegradability and environmental soundness. In this study, a pH stage-controlled process of fermentation of rhamnolipids by Pseudomonas aeruginosa O-2-2 was established. A yield of 24.06 g/L in batch fermentation was achieved in a 5-L fermentor via the optimization of stirring speed. By controlling pH, rhamnolipid production was increased to 28.8 g/L, an improvement of 19.7%, and more substrate was converted to rhamnolipids rather than to biomass. Fermentation kinetics models for cell growth, product synthesis and substrate consumption based on the pH stage-controlled fermentation indicated that rhamnolipid production could be further improved by fed-batch fermentation. Rhamnolipid production reached 70.56 g/L, an improvement of 193%, in the pH stage-controlled fed-batch fermentation when the stirring speeds was controlled at 500 rpm and the fermentation temperature was maintained at 30 degrees C. (C) 2012 Elsevier Ltd. All rights reserved.