Proteins involved in lipid translocation in eukaryotic cells
Proteins involved in lipid translocation in eukaryotic cells
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DOI：
10.1016/j.chemphyslip.2006.02.007
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Bryde, Susanne
中科院分区：
文献类型：
--
作者：
Devaux, Philippe F.;Lopez-Montero, Ivan;Bryde, Susanne
自从1984年首次发现人红细胞膜上存在依赖于ATP的脂质转位以来，已有大量证据表明真核细胞膜中存在多种ATPase转运脂质。它们包括P型ATPase，参与从外质小叶到胞质小叶的内向脂运输，以及ABC蛋白，参与向外运输。还有一些不依赖于三磷酸腺苷的蛋白质可以催化脂质的双向传递。酵母细胞中有五种与脂质转运有关的P型ATPase的基因特征，这表明有几种蛋白质具有部分冗余的活性，负责调节脂质的不对称性。然而，单个酵母蛋白的表达和纯化仍然不足以在脂质体中进行重建实验。在这篇综述中，我们想对目前关于鉴定和纯化可能与脂质易位有关的蛋白质的研究工作进行综述。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Since the first discovery of ATP-dependent translocation of lipids in the human erythrocyte membrane in 1984, there has been Much evidence of the existence of various ATPases translocating lipids in eukaryotic cell membranes. They include P-type ATPases involved in inwards lipid transport from the exoplasmic leaflet to the cytosolic leaflet and ABC proteins involved in outwards transport. There are also ATP-independent proteins that catalyze the passage of lipids in both directions. Five P-type ATPase involved in lipid transport have been genetically characterized in yeast cells, suggesting a pool of several proteins with partially redundant activities responsible for the regulation of lipid asymmetry. However, expression and purification of individual yeast proteins is still insufficient to allow reconstitution experiments in liposomes. In this review, we want to give an overview over current investigation efforts about the identification and purification of proteins that may be involved in lipid translocation. (c) 2006 Elsevier Ireland Ltd. All rights reserved.