Cellular dynamics of the hormonal and developmental induction of hepatic alpha 2u globulin as demonstrated by immunocytochemistry and specific mRNA monitoring.
Cellular dynamics of the hormonal and developmental induction of hepatic alpha 2u globulin as demonstrated by immunocytochemistry and specific mRNA monitoring.
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免疫细胞化学和特异性 mRNA 监测证明了肝 α2u 球蛋白的激素和发育诱导的细胞动力学。
DOI：
10.1002/aja.1001650209
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Feigelson,P
中科院分区：
文献类型：
--
作者：
Antakly,T;Lynch,KR;Nakhasi,HL;Feigelson,P
这项免疫过氧化物酶研究确定了在雄性个体发育和其他内分泌状态下合成α 2u球蛋白的肝细胞，其中α 2u球蛋白的合成受到调节。为了将生化参数与免疫细胞化学相关联，通过克隆的α 2 u球蛋白cDNA探针的北方印迹杂交和Rot分析评价α 2 u球蛋白mRNA（α 2 u mRNA）序列，并通过无细胞翻译测定该mRNA的功能水平。α 2 u球蛋白及其mRNA在肝脏中检测到后，雄性大鼠进入青春期。在其发育和激素诱导，观察到显着的异质性，小叶中心肝细胞表现出最强的染色，而门静脉周围肝细胞没有，或弱染色，这种蛋白质。小叶中心和门静脉周围肝细胞之间的这种差异在发育过程中得到增强。成熟雄性大鼠表现出最强的染色反应和最高水平的肝α 2u球蛋白mRNA。几乎所有成年雄性的肝细胞都被染色;然而，小叶中心的肝细胞比门静脉周围的肝细胞染色明显更深。雌性大鼠肝脏中没有这种蛋白质及其mRNA。对去势雌性大鼠给予雄激素或对肾上腺切除雄性大鼠给予氢化可的松后，α 2u球蛋白mRNA水平以及小叶中心α 2u球蛋白染色肝细胞的数量和强度平行增加。垂体切除后，几乎没有α 2u球蛋白可以定位，同样，它的mRNA是不可检测的。然而，当垂体切除的成年雄性大鼠用生长激素、睾酮、甲状腺激素和氢化可的松处理时，肝脏免疫细胞化学染色以及α 2u球蛋白mRNA序列和功能性α 2u球蛋白mRNA的水平，以及α 2u球蛋白的体内生物合成速率，变得与完整的成熟雄性大鼠相当。这些研究表明肝细胞之间的功能异质性，这似乎是由它们在肝小叶内的解剖位置决定的。此外，α 2u球蛋白的激素和发育诱导涉及参与其合成的肝细胞数量的增加和已定型细胞中其合成速率的增加。这些事件伴随肝脏α 2u球蛋白mRNA水平的同时升高。
This immunoperoxidase study identifies the hepatocytes that synthesize alpha 2u globulin during male ontogeny and other endocrine states wherein its synthesis is modulated. To correlate biochemical parameters with the immunocytochemistry, alpha 2u globulin mRNA (alpha 2u mRNA) sequences were evaluated by Northern blot hybridization and Rot analysis to a cloned alpha 2u globulin cDNA probe and the functional level of this mRNA measured by cell‐free translation. Alpha 2u globulin and its mRNA were detectable in the liver only after male rats reached puberty. During its developmental and hormonal induction, marked heterogeneity was observed; centrolobular hepatocytes showed the strongest staining, whereas periportal hepatocytes were devoid of, or weakly stained for, this protein. This difference between centrolobular and periportal hepatocytes was enhanced during development. Mature male rats exhibited the strongest staining reaction and the highest levels of hepatic alpha 2u globulin mRNA. Almost all hepatocytes in the adult male were stained; however, centrolobular ones were remarkably more stained than periportal ones. Female rat liver was devoid of this protein and of its mRNA. Following androgen administration to castrated female rats, or hydrocortisone administration to adrenalectomized male rats, the alpha 2u globulin mRNA level as well as the number and intensity of centrolobular alpha‐2u‐globulin‐staining hepatocytes were increased in parallel. Following hypophysectomy, almost no alpha 2u globulin could be localized; likewise its mRNA was undetectable. However, when hypophysectomized adult male rats were treated with growth hormone, testosterone, thyroid hormone, and hydrocortisone, the hepatic immunocytochemical staining as well as the level of alpha 2u globulin mRNA sequences and of functional alpha 2u globulin mRNA, and the in vivo biosynthetic rate of alpha 2u globulin, became comparable to that of intact mature male rats. These studies indicate functional heterogeneity among hepatocytes which seems to be determined by their anatomical location within the hepatic lobule. Furthermore, hormonal and developmental induction of alpha 2u globulin involves both an increase in the number of hepatocytes committed to its synthesis and an increase in the rate of its synthesis in already committed cells. These events are accompanied by a concurrent increase in the hepatic alpha 2u globulin mRNA level.
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生长激素在 α2u 球蛋白合成中的作用：重新审视
影响因子：
64.5
作者：
K. Lynch;K. Dolan;H. Nakhasi;R. Unterman;P. Feigelson
通讯作者：
P. Feigelson
肝癌中 α2u 球蛋白基因结构、转录和 mRNA 翻译的修饰。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nakhasi,HL;Lynch,KR;Dolan,KP;Unterman,R;Antakly,T;Feigelson,P
通讯作者：
Feigelson,P
雄性大鼠肝细胞核中α2u-球蛋白基因的体外转录。
影响因子：
2.9
作者：
K. M. Chan;D. T. Kurtz;P. Feigelson
通讯作者：
P. Feigelson
分离肝细胞中 α2u 球蛋白合成及其 mRNA 的激素控制
影响因子：
5.2
作者：
Ching;P. Feigelson
通讯作者：
P. Feigelson
用组织免疫学方法观察到的含白蛋白肝细胞的随机排列。
DOI：
10.1002/ar.1091970208
发表时间：
1980
期刊：
The Anatomical Record
影响因子：
--
作者：
A. LeBouton;J. Peters Masse
通讯作者：
J. Peters Masse