MEMBRANE PERMEABLE FLUOROGENIC RHODAMINE SUBSTRATES FOR SELECTIVE DETERMINATION OF CATHEPSIN-L
MEMBRANE PERMEABLE FLUOROGENIC RHODAMINE SUBSTRATES FOR SELECTIVE DETERMINATION OF CATHEPSIN-L
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DOI：
10.1515/bchm3.1992.373.2.433
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
MACHLEIDT, W
中科院分区：
文献类型：
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作者：
ASSFALGMACHLEIDT, I;ROTHE, G;MACHLEIDT, W
二肽基罗丹明二胺底物（z - ph - arg）2-R110和（Z-Arg-Arg）2-R110对组织蛋白酶L的选择性比对组织蛋白酶B的选择性高820倍和360倍，从而在组织蛋白酶B具有高活性的情况下灵敏地测定组织蛋白酶L的活性。用细胞裂解物获得的结果表明，用这些底物流式细胞术检测重要巨噬细胞的半胱氨酸蛋白酶活性主要是由组织蛋白酶L引起的。
The dipeptidyl rhodamine diamide substrates (Z-Phe-Arg)2-R110 and (Z-Arg-Arg)2-R110 are 820- and 360-fold more selective for cathepsin L than for cathepsin B allowing a sensitive determination of cathepsin L activity in the presence of high activity of cathepsin B. The results obtained with cell lysates suggest that the cysteine proteinase activity of vital macrophages detected by flow cytometry with these substrates is mainly due to cathepsin L.