Lymphocyte membrane lectins. Direct visualization by the use of fluoresceinyl‐glycosylated cytochemical markers
Lymphocyte membrane lectins. Direct visualization by the use of fluoresceinyl‐glycosylated cytochemical markers
复制标题
使用荧光素糖基化细胞化学标记物直接观察淋巴细胞膜凝集素。
DOI：
10.1016/0014-5793(79)80984-9
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发表时间：
1979
期刊：
影响因子：
3.5
通讯作者：
M. Monsigny
中科院分区：
文献类型：
--
作者：
C. Kieda;A. Roche;F. Delmotte;M. Monsigny
已知凝集素选择性地结合碳水化合物部分并且作为水溶性蛋白质存在于多种生物体中[1 -3]。最近，发现凝集素也作为各种细胞的膜组分存在[4，5]，特别是小鼠淋巴细胞[6]。迄今为止，膜凝集素主要通过分离膜的生化方法来表征。我们在此描述了通过使用糖基化细胞化学标记物的荧光素基衍生物，原位直接观察膜凝集素[7，8]。
Lectins are known to bind selectively carbohydrate moieties and to be present as water-soluble proteins in a large variety of organisms [l-3]. Recently, it was found that lectins were also present as membrane components of various cells [4, 5] and especially of mouse lymph~ ytes [6]. So far, membrane lectins have been mainly characterized by biochemical methods from isolated membranes. We describe here the direct visualization of membrane lectins in situ, by the use of fluoresceinyl derivatives of glycosylated cytochemical markers [7, 8].