Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy
Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy
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DOI：
10.1159/000452102
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Kosnik, Mitja
中科院分区：
文献类型：
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作者：
Korosec, Peter;Silar, Mira;Kosnik, Mitja
背景：我们试图确定在变应性鼻炎和过敏性膜翅目毒液超敏反应中，嗜碱性细胞变应原敏感性是否可以通过被动的IgE增敏传递给供体嗜碱性细胞。方法：我们研究了15名黄蜂毒液、19名草粉和2名屋尘螨过敏患者、2名健康献血员和8名黄蜂毒液过敏献血员。在所有受试者中，我们首先评估了嗜碱性细胞对黄蜂毒液、草花粉或屋尘螨变应原的初始反应。然后剥离捐赠者的嗜碱性粒细胞，用不同患者的血清IgE致敏，并用相应的过敏原攻击。然后将供体嗜碱性粒细胞的CD63反应与最初的嗜碱性细胞反应进行比较。结果：在黄蜂毒液过敏性受试者中，IgE转移不能反映嗜碱性粒细胞对变应原的初始敏感性，因为嗜碱性细胞敏感度高或低的受试者的毒液IgE能诱导健康供者嗜碱性粒细胞产生类似的反应性。此外，反之亦然，当我们用不同的黄蜂毒或屋尘螨IgE致敏黄蜂毒液过敏性个体的供体嗜碱性粒细胞时，我们证明了他们的反应可以由他们最初的嗜碱性变应原敏感性来预测。在鼻炎变态反应模型中，IgE转移与患者最初的嗜碱性粒细胞反应性相关，因为高嗜碱性粒细胞过敏原敏感性的受试者的草花粉IgE诱导供者嗜碱性粒细胞的反应性显著高于最初碱性嗜碱性粒细胞过敏原敏感性低的受试者的IgE。结论：我们的结果表明，用流式细胞仪CD63分析评估嗜碱性粒细胞变应原敏感性取决于两个不同的贡献因素。在过敏性膜翅目过敏中，主要因素是细胞内的敏感性，而在花粉过敏中，主要因素是细胞表面的过敏原特异性IgE。(C)2016年S.Karger AG，巴塞尔
Background: We sought to determine whether basophil-allergen sensitivity could be transferred to donor basophils by passive IgE sensitisation in allergic rhinitis and anaphylactic Hymenoptera venom hypersensitivity. Methods: We studied 15 wasp venom-, 19 grass pollen-and 2 house dust mite-allergic patients, 2 healthy donors, and 8 wasp venom-allergic donors. In all subjects, we first evaluated the initial basophil response to wasp venom, grass pollen, or house dust mite allergen. Donor basophils were then stripped, sensitised with the different patients' serum IgE, and challenged with the corresponding allergen. The CD63 response of donor basophils was then compared with initial basophil responses. Results: In wasp venom-allergic subjects, the IgE transfer did not reflect the initial basophil-allergen sensitivity, because the venom IgE of subjects with high or low basophil sensitivity induced comparable responsiveness in healthy donor basophils. Furthermore, vice versa, when we sensitised the donor basophils of wasp venom-allergic individuals with different wasp venom or house dust mite IgE, we demonstrated that their response was predictable by their initial basophil allergen sensitivity. In the rhinitis allergy model, the IgE transfer correlated with the patients' initial basophil responsiveness because the grass pollen IgE of the subjects with high basophil allergen sensitivity induced significantly higher responsiveness of donor basophils than the IgE of subjects with initially low basophil allergen sensitivity. Conclusions: Our results suggest that basophil allergen sensitivity evaluated by flow-cytometric CD63 analysis depends on two distinct contribution factors. In anaphylactic Hymenoptera allergy, the major factor was intrinsic cellular sensitivity, whereas in pollen allergy, the major factor was allergen-specific IgE on the cell surface. (C) 2016 S. Karger AG, Basel