Spatial and temporal expression analysis of defense-related genes in soybean cultivars with different levels of partial resistance to Phytophthora sojae
Spatial and temporal expression analysis of defense-related genes in soybean cultivars with different levels of partial resistance to Phytophthora sojae
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DOI：
10.1016/j.pmpp.2005.07.001
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发表时间：
2005-05-01
影响因子：
2.7
通讯作者：
Dorrance, AE
中科院分区：
文献类型：
--
作者：
Vega-Sánchez, ME;Redinbaugh, MG;Dorrance, AE
大豆对大豆疫霉部分抗性的分子机制和防御反应尚不清楚。在这项研究中，我们研究了防御基因的表达与部分抗性之间的相关性。首先，为了确定防御相关基因的组成型表达水平是否与对大豆疫霉的部分抗性相关，对具有低、中、高水平部分抗性的14个品种中的7个防御相关基因进行了北方印迹分析。平均损伤长度和防御相关基因的平均组成型mRNA信号之间的Pearson相关性表明与对大豆疫霉的部分抗性没有显著关联。这些结果表明，与防御相关mRNA表达水平相关的机制可能更好地解释了大豆对大豆疫霉部分抗性的变化。第二，以Conrad（高水平的部分抗性）和OX 20-8（Rps 1a，低水平的部分抗性）两个大豆品种为材料，通过空间和时间过程感染试验，监测了4种防御相关转录本在感染过程中的积累。从接种大豆疫霉后0、6、12、24、48和72 h（hai）在接种部位下方、处和上方收获的根切片分离mRNA用于北方印迹分析。大豆疫霉和大豆肌动蛋白cDNA被用作感染的根切片中的探针，以估计RNA的相对比例。Conrad和OX 20-8的三个根段中大豆疫霉定殖后大豆和大豆疫霉肌动蛋白的差异mRNA积累模式表明，有效的病变限制机制主要发生在上部根段。PR 1a，基质金属蛋白酶（MMP）和碱性过氧化物酶（IPER）在接种部位的转录水平;和IPER以上的接种部位在72海与部分抗性水平较高的康拉德显着较高。这些结果表明，PR 1a、MMP、IPER和β-1，3-内切葡聚糖酶（EGL）mRNA的积累可能是大豆对大豆疫霉部分抗性的原因之一。(c)2005爱思唯尔有限公司保留所有权利。
The molecular mechanisms and the defense responses associated with partial resistance to Phytophthora sojae in soybean are unknown. In this study, we examined correlations between the expression of defense genes with partial resistance. First, to determine whether constitutive levels of expression of defense-related genes correlated with partial resistance to P. sojae, northern blot analysis of seven defense-related genes in 14 cultivars with low, moderate and high levels of partial resistance was performed. Pearson's correlations between mean lesion length and mean constitutive mRNA signals for defense-related genes showed no significant association to partial resistance to P. sojae. These results suggested that mechanisms linked to defense-related mRNA levels expressed during infection might better explain variations in partial resistance to P. sojae in soybean.Second, accumulation of four defense-related transcripts during infection was monitored in a spatial, time-course infection assay with two soybean cultivars, Conrad (high level of partial resistance) and OX 20-8 (Rps1a, low level of partial resistance). mRNA was isolated for Northern blot analysis from root sections harvested below, at, and above the inoculation site at 0, 6, 12, 24, 48 and 72 h after inoculation (hai) with P. sojae. P. sojae and soybean actin cDNAs were used as probes in the infected root sections to estimate relative proportions of RNA. Differential mRNA accumulation patterns for both soybean and P. sojae actin following P. sojae colonization in the three root sections of Conrad and OX 20-8 suggested that effective lesion-limiting mechanisms occurred primarily in the upper root section. Transcript levels for PR1a, matrix metalloproteinase (MMP) and basic peroxidase (IPER) at the inoculation site; and IPER above the inoculation site at 72 hai were significantly higher in Conrad with higher levels of partial resistance. Our results suggest that defense responses associated with accumulation of PR1a, MMP, IPER and beta-1,3-endoglucanase (EGL) mRNAs may contribute to the partial resistance response to P. sojae in soybean. (c) 2005 Elsevier Ltd. All rights reserved.