Effects of transient local heating of spatially and spectrally heterogeneous biotissue by short laser pulses
Effects of transient local heating of spatially and spectrally heterogeneous biotissue by short laser pulses
复制标题
短激光脉冲对空间和光谱异质生物组织的瞬时局部加热的影响
DOI:
10.1007/bf02457180
复制
发表时间:
1991
期刊:
影响因子:
--
通讯作者:
V. Letokhov
中科院分区:
文献类型:
--
作者:
V. Letokhov
SummaryA new laser method for the spectroscopy of locally absorbing microvolumes much smaller than the radiation wavelength in size is discussed. Such an absorption is characteristic of biotissues and other biological media. The method allows data to be obtained on the size of local microvolumes absorbing at the radiation wavelength. It is based on the possibility of transient overheating of the microvolumes by means of an ultrashort pulse with a duration much shorter than the time it takes to heat to diffuse from the microvolumes. The pulse-heated microvolumes must have an altered refractive index leading to an additional scatter of another probe laser pulse, that is made to irradiate the medium under study after some delay. Besides, the locally heated microvolumes will have temperature-altered fluorescence induced either by the heating laser pulse or by an additional probe pulse at another wavelength more suitable for fluorescence excitation.