Biological hydrogen production measured in batch anaerobic respirometers
Biological hydrogen production measured in batch anaerobic respirometers
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DOI：
10.1021/es015783i
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发表时间：
2002-06-01
影响因子：
11.4
通讯作者：
Van Ginkel, S
中科院分区：
文献类型：
--
作者：
Logan, BE;Oh, SE;Van Ginkel, S
从不同底物的发酵的生物生产氢进行了检查，在批量测试中使用热休克混合培养物与两种技术：间歇压力释放法（欧文法）和连续气体释放法，使用气泡测量装置（呼吸法）。在其他条件相同的情况下，呼吸法导致从葡萄糖产生的氢气比欧文法多43%。使用Owen方案的葡萄糖到氢气的较低转化率可能是由气密瓶中高分压的氢化酶活性抑制产生的，但这不能使用热力学/速率抑制分析来证明。在呼吸法中，顶部空间中的总压力从未超过环境压力，并且氢气通常占顶部空间气体的62%。在不同时间和储存长达一个月的热休克土壤一致获得高转换效率。在对数生长期间，葡萄糖生长的培养物的氢气组成始终在60-64%的范围内，但在静止培养物中下降。总体而言，基于最大4摩尔氢/摩尔葡萄糖的假设，葡萄糖培养物的氢转化效率为23%。蔗糖的氢转化效率相似（23%），糖蜜的氢转化效率略低（15%），但乳酸盐（0.50%）和纤维素（0.075%）的氢转化效率要低得多。
The biological production of hydrogen from the fermentation of different substrates was examined in batch tests using heat-shocked mixed cultures with two techniques: an intermittent pressure release method (Owen method) and a continuous gas release method using a bubble measurement device (respirometric method). Under otherwise identical conditions, the respirometric method resulted in the production of 43% more hydrogen gas from glucose than the Owen method. The lower conversion of glucose to hydrogen using the Owen protocol may have been produced by repression of hydrogenase activity from high partial pressures in the gastight bottles, but this could not be proven using a thermodynamic/rate inhibition analysis. In the respirometric method, total pressure in the headspace never exceeded ambient pressure, and hydrogen typically composed as much as 62% of the headspace gas. High conversion efficiencies were consistently obtained with heat-shocked soils taken at different times and those stored for up to a month. Hydrogen gas composition was consistently in the range of 60-64% for glucose-grown cultures during logarithmic growth but declined in stationary cultures. Overall, hydrogen conversion efficiencies for glucose cultures were 23% based on the assumption of a maximum of 4 mol of hydrogen/ mol of glucose. Hydrogen conversion efficiencies were similar for sucrose (23%) and somewhat lower for molasses (15%) but were much lower for lactate (0.50%) and cellulose (0.075%).