1H NMR-based metabolomic study of the effects of flavonoids on citrinin production by Monascus
1H NMR-based metabolomic study of the effects of flavonoids on citrinin production by Monascus
复制标题
基于 1 H NMR 的代谢组学研究黄酮类化合物对红曲菌产生橘霉素的影响
DOI：
10.1016/j.foodres.2020.109532
复制
发表时间：
2020-11-01
影响因子：
8.1
通讯作者：
Huang, Zhibing
中科院分区：
文献类型：
--
作者：
He, Shanshan;Wang, Yanling;Huang, Zhibing
红曲霉菌由紫红色霉菌组成。从这些物种中可以获得各种化合物，包括他汀类药物和食品安全的黄色、红色和橙色色素。然而，次生代谢物桔霉素是一种真菌毒素，在生长的后期产生。为了安全地使用红曲色素，应减少桔霉素的生物合成。幸运的是，这可以通过添加黄酮类(染料木素、大豆苷元、芹菜素和山奈酚)来实现。然而，这些黄酮类化合物对其他代谢物的影响尚不清楚。在这里，我们报告了一种基于H-1核磁共振的多变量代谢组学分析，研究了黄酮类化合物对红曲霉菌毒素桔霉素生产的影响。检测到15种与赖氨酸和精氨酸生物合成有关的代谢物，以及丙氨酸、天冬氨酸、谷氨酸、生物素、精氨酸、脯氨酸和谷胱甘肽的代谢产物。谷氨酸、天冬氨酸、生物素和2-磷酸甘油含量的降低表明它们与桔霉素的还原机制有关。本研究确定了红曲霉中桔霉素的产生途径，并将有助于开发桔霉素的控制方法。
Monascus comprises purple-red molds. Various compounds can be obtained from these species, including statins and food-safe yellow, red, and orange pigments. However, the secondary metabolite citrinin, a mycotoxin, is produced during the late stages of growth. Citrinin biosynthesis should be reduced to apply Monascus pigments safely. Fortunately, this can be achieved by the addition of flavonoids (genistein, daidzein, apigenin, and kaempferol). However, the effects of these flavonoids on other metabolites remain unknown. Here, we report a H-1 NMR-based multivariate metabolomic analysis of the effects of flavonoids on mycotoxin citrinin production by Monascus. Fifteen metabolites involved in lysine and arginine biosynthesis and alanine, aspartate, glutamate, biotin, arginine, proline, and glutathione metabolism were detected. The reduction in glutamate, aspartate, biotin, and 2-phosphoglycerate content suggested their association with the citrinin reduction mechanism. This study identifies the citrinin production pathway in Monascus and will aid in the development of citrinin-control methods.